A Metabolomics-based Study Of Serum Metabolites In Children With Purpuric Nephritis

Purpose: The use of liquid chromatography-mass spectrometry(LC-MS)to analyze and study the serum metabolites of children with purpuric nephritis and healthy children,purpuric nephritis,heat toxin injury syndrome and Qi deficiency and blood stasis syndrome in children with purpuric nephritis and Among them,the material basis research of medical syndrome type provides reference.Material and method:1.Sample collection and grouping: In the outpatient clinic of the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,15 children with purpuric nephritis who met the inclusion criteria were selected as the purpura kidney group(N),including 5 cases of Qi deficiency and blood stasis syndrome(N1),heat toxin 10 cases of collateral injury syndrome(N2).Collect the venous blood samples of the above-mentioned children,and recruit 10 healthy children as the control group(H),inform their parents in detail of the purpose of the research project they participated in,and sign informed consent.This research plan was approved by the Medical Ethics Committee of Liaoning University of Traditional Chinese Medicine.2.Use liquid chromatography-mass spectrometry technology to perform comprehensive scanning and detection of samples,combined with metabolomics analysis methods to explore and analyze serum metabolites,and preprocess the obtained raw data and import them into relevant software to analyze the obtained small Qualitative and quantitative analysis of molecular compounds.Use multivariate statistics and pattern discriminant analysis methods to find statistically significant differences in metabolites between children with purpuric nephritis and healthy children,purpuric nephritis,heat toxin and collateral injury syndrome,and Qi deficiency and blood stasis syndrome;the difference is analyzed through the metabonomics database Screening and comparison of sex metabolites,preliminary identification of relevant biomarkers,and analysis of metabolic pathways.Results:1.In the PLS-DA and OPLS-DA model discriminant chart,the samples of the purpura kidney group and the healthy control group are clustered within the group respectively;the samples of the purpura kidney group and the healthy control group are respectively distributed on both sides of the central axis,and the samples of the two groups are in The obvious distinction in the figure shows that there is a significant difference in metabolic status between children with purpuric nephritis and healthy children.2.In the PLS-DA and OPLS-DA model discriminant chart,the heat toxin injury group and the Qi deficiency and blood stasis group are also clustered within each group;the heat toxin injury group and the Qi deficiency and blood stasis group are distributed on both sides of the central axis,The two groups of samples are clearly distinguished in the figure,indicating that there are also significant differences in the metabolic status of purpuric nephritis,heat toxin and collateral injury syndrome and Qi deficiency and blood stasis syndrome.3.A comparative study of serum metabolites between the purpura kidney group and the healthy control group found that 7 different metabolites were identified,namely:trihydroxyisobutyrate,citicoline,phenylalanine,and deoxyadenosine Phosphoric acid,arginine,proline,tyrosine.Among them,these 7 different metabolites are related to the degradation of valine,leucine and isoleucine,glycerophospholipid metabolism,phenylalanine metabolism,purine metabolism,arginine and proline metabolism,and tyrosine Metabolism and other metabolic pathways are involved.4.A comparative study of serum metabolites in children's purpuric nephritis heat toxin injury group and Qi deficiency and blood stasis group found that 4 different metabolites were identified,namely: tryptophan,glycine,tyrosine,and citicoline.These four different metabolites are related to phenylalanine,tyrosine and tryptophan biosynthesis,glycerophospholipid metabolism,glycine,serine and threonine metabolism,tyrosine metabolism and other metabolic pathways.5.Purpuric nephritis and the healthy control group,the purpuric nephritis heat toxin injury group and the Qi deficiency and blood stasis group have two identical differential metabolites,namely citicoline and tyrosine.Conclusion:1.Comparing children with purpuric nephritis and healthy controls,there are 7 different metabolites,trihydroxyisobutyric acid,citicoline,phenylalanine,deoxyadenosine triphosphate,arginine,proline Acid,tyrosine.Involve 6 metabolic pathways: degradation of valine,leucine and isoleucine,glycerophospholipid metabolism,phenylalanine metabolism,purine metabolism,arginine and proline metabolism,tyrosine metabolism It is related to the metabolism of protein,lipids,carbohydrates,etc.,provides a theoretical basis for the early diagnosis of purpuric nephritis,and perfects the TCM diagnosis system of purpuric nephritis.2.Compared with the qi deficiency and blood stasis group,there are 4 different metabolites in the purpuric nephritis heat toxin injury group: tryptophan,glycine,tyrosine,and citicoline,involving 4 metabolic pathways: phenylpropanol The biosynthesis of acid,tyrosine and tryptophan,the metabolism of glycerophospholipid,the metabolism of glycine,serine and threonine,and the metabolism of tyrosine can provide references for the study of the material basis of TCM syndromes in children with purpuric nephritis.