| Purpose:Objective to explore the protective mechanism of Buyang Huanwu Decoction on retinal blue light injury in rats.Material and method:In this experiment,SD rats were divided into five groups with five rats in each group: blank control group(N),model control group(M),Buyang Huanwu Decoction low concentration group(A),Buyang Huanwu Decoction medium concentration group(B)and Buyang Huanwu Decoction high concentration group(C).25 rats were used to establish atrophic age-related macular degeneration(AMD)model by 12 hour alternating light and dark irradiation with 8000 K blue light of 480 nm wavelength.Each group of rats were examined by fundus photography and dark field flash ERG.The period of establishing AMD model was 14 days,and the drug was given one week before modeling,and the period of administration was 21 days.On the 21 st day of administration,fundus photography and ERG detection were carried out to count the changes of retinal cup disc ratio and ERG amplitude;on the 22 nd day,the eyeballs of rats were taken with a certain length of optic nerve,frozen and paraffin embedded to make sections;he staining method was used to detect the retinal thickness and the number of retinal ganglion cells in each group;toluidine blue staining was used to detect the optic nerve injury in each group;immunofluorescence staining was used to detect the optic nerve injury in each group The expression of Nrf2 was detected by fluorescence staining,and the apoptosis of retinal cells was detected by TUNEL.Results:After 21 days of administration,compared with the blank control group,scattered hyaline warts and neovascularization appeared in the retinal fundus of the model control group,and the ratio of cup to disc increased significantly(P < 0.01).Compared with the model group,the ratio of cup to disc in the high concentration group was significantly lower(P < 0.05).The results of dark field flash ERG showed that the amplitudes of a and B waves in the retina of rats after 14 days of blue light irradiation decreased significantly compared with the blank control group(P < 0.001).Compared with the model control group,b wave in the high concentration group was increased(P < 0.05).The retinal thickness was measured by HE staining,and the results showed that the retinal damage in the high concentration group was less than that in the model group(P < 0.05).The number of retinal ganglion cells in the middle concentration group was higher than that in the model group(P < 0.05).Compared with the model group,the number of ganglion cells in the high concentration group was significantly increased(P < 0.01).Toluidine blue staining was used to detect the optic nerve injury.Compared with the model group,the number of axons in the medium concentration group and the high concentration group increased(P < 0.01).The results of Nrf2 immunofluorescence staining showed that the fluorescence intensity of the medium concentration group was higher than that of the model control group(P < 0.01).The expression of Nrf2 in retina of high concentration group was also significantly higher than that of model control group(P < 0.001).Conclusion:Blue light irradiation can cause retinal light damage in rats.Buyang Huanwu Decoction at high,medium and low concentrations has protective effect on retinal damage after 21 days.Buyang Huanwu Decoction at medium concentration can reduce retinal ganglion cell damage,reduce optic nerve damage and increase the expression of Nrf2.High concentration of Buyang Huanwu Decoction can improve the retinal cell damage caused by light,increase the retinal thickness,improve the retinal morphology,protect the optic nerve,and increase the expression of Nrf2 in the retina.It is proved that Buyang Huanwu Decoction can reduce the retinal damage caused by blue light and protect the retina from oxidative damage in rats. |
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