The Clinical Diagnostic Value Of Cell Membrane Surface Markers And Cytokines In 80 Cases Of Infection And Severe Infection Samples Was Explored By Flow Cytometry

[Objective]To evaluate the clinical application value of CD64 of neutrophils,CD 14 of monocytes,HLA-DR of monocytes,interleukin-2,interleukin-4,interleukin-6,interleukin-8,interleukin-1?,interleukin-17,interleukin-10,tumor necrosis factor,?-interferon in infection and severe infection by flow cytometry.[Methods]EDTA anticoagulant peripheral venous blood from 100 patients and 90 patients from outpatient and inpatients of Peking Union Medical College Hospital were selected for cell membrane surface markers and paraments to assess the changes of each index in the infected and non-infected groups;By the ROC curve of the subjects to analyze the effectiveness of each index in diagnosis of infection and severe infection and the diagnosis of the best cutoff value and the corresponding sensitivity and specificity;By the severe infection blood flow infection patient samples as negative results of the indicators of flora analysis,and non-infected patients as positive results of the indicators of the case analysis of the degree of compliance.[Results]The infection groups of each index were significantly different from those of non-infection groups.Interleukin-6,Interleukin-8 and Interleukin-10 are the most obvious differences;Interleukin-8 is the most effective in the clinical diagnosis of infection and severe infection.Interleukin-6 is effective in the diagnosis of severe infection.At the threshold of diagnosis,Interleukin-8 and interferon-? have better sensitivity in diagnosis of infection and severe infection.Among them,the sensitivity of Interferon-? to diagnosis of severe infection can reach 100%.Interleukin-4 is more sensitive in the diagnosis of severe infection.The specificity of HLA-DR and Interleukin-6 in diagnosis of infection and severe infection are better.Maybe the specificity of interleukin-2 in diagnosis of severe infection is better;Compared with Gram-negative bacilli,maybe monocyte CD 14 has higer diagnostic ability than Gram-positive cocci.And maybe the expression level returned to normal slowly during the convalescence of infection.Interleukin-2 may be poorly detected in Gram-negative bacteria.Interleukin-6 may not be fully covered by Gram-negative bacteria.The indicators of cancer patients are prone to false positive.Maybe CD 14 and Interleukin-1? infection improved are returned to normal slowly.[Conclusions]All index have a certain detection effect on severe infection,and the diagnostic efficacy of HLA-DR and IL-8 of severe infection are better.The combined detection of several markers can improve the clinical diagnostic efficacy.