Astragaloside IV Regulates The SDF-1?/CXCR4 Axis On Endothelial Progenitor Cell Migration Under High Glucose

Objective: This project was conducted based on the relationship between Astragaloside ?(AS-?)on the mobilization and migration of Endothelial progenitor cells(EPCs)and the SDF-1a/CXCR4 signal axis in a high-glucose environment,thus providing a theoretical basis for the application of AS-? in the clinical treatment of diabetic vascular disease.Methods: Firstly,to take the postpartum human umbilical vein and separate human umbilical vein endothelial cell(HUVEC)and EPCs from it.HUVEC and EPCs were cultured in vitro.EPCs were identified by CD31 antibody combined with DAPI nuclear staining + FITC-UEA-I and Dil-ac-LDL dual fluorescent staining;while HUVEC was identified by combined cell-marker v WF combined with DAPI nuclear staining.Secondly,to measure the secretions of SDF-1? and CXCR4 in HUVEC and EPCs under normal physiological conditions in high glucose environment.And this research will examine the effects of different concentrations of AS-? intervention on the secretion of SDF-1a and CXCR4 in HUVEC and EPCs at different time periods under the two conditions.Afterwards,the optimal concentration of AS-? to promote HUVEC to secrete SDF-1a and EPCs to secrete CXCR4 will be determined respectively in terms of scope and time.Then the author will collect the cell supernatant when astragaloside ? promotes HUVEC to secret SDF-1? optimally for use,and determine the concentration of SDF-1? at this time.Thirdly,to use the optimal concentration of astragaloside ? to cultivate the EPCs under physiological conditions and the high glucose environment at the optimal time to promote the secretion of CXCR.Then the migration ability of EPCs will be detected with the intervention of the SDF-1a/CXCR4 specific inhibitor AMD3100 under the infulence of the cell supernatant containing SDF-1? and the same concentration of SDF-1? respectively.Results: 1.The experimental group(after the intervention of different concentrations of AS-?)high glucose-impaired EPCs,HUVEC expression SDF-1?,CXCR4 were significantly higher than the control group,the difference was statistically significant(P<0.05).2.The best concentration of AS-? to promote the secretion of CXCR4 in EPCs under high glucose environment is 50 mg/L,and the best time is 48 h.The optimal concentration of AS-? to promote the secretion of SDF-1? in HUVEC under high glucose environment is 100 mg/L,and the optimal time is 24h;the concentration of SDF-1? in the cell supernatant is 2ug/ml at this time;3.Compared with the negative control group(AS-?+HG+HUVEC cell supernatant + AMD3100),the experimental group(AS-?+HG+HUVEC cell supernatant group)and the positive control group(AS-?+HG+SDF)-1?),the cell migration rate was significantly increased,which was statistically significant(P<0.01).Compared with the normal group,there was no significant difference in the relative width of cell migration between the experimental group and the normal group(P?0.05).Conclusion: 1.Different concentrations of astragaloside ? can improve the secretion of SDF-1a and CXCR4 in HUVEC and EPCs in high glucose environment to varying degrees.2.The optimal concentration of astragaloside ? to promote the secretion of CXCR4 in EPCs in a high glucose environment is 50 mg/L,and the optimal time is48h;the optimal concentration of astragaloside ? to promote the secretion of SDF-1?in HUVECs in a high glucose environment is 100 mg /L,the best time is 24 h.3.Astragaloside ? can improve the migration and mobilization ability of EPCs in high glucose environment by adjusting the SDF-1?/CXCR4 axis.