Establishment And Functional Analysis Of Proteome Map Of Normal Mouse White Adipose Tissue

Backgound:White adipose tissue secretes hormones and cytokines to regulate energy intake,energy expenditure,and carbohydrate and lipid metabolism.These proteins are considered a valuable resource in disease research.Therefore,a comprehensive profile of the WAT proteome will provide a basic understanding of WAT function and benefit disease studies.Method:In this study,6 normal C57BL/6J mice was applied to collect 300 mg gonadal WAT to generate a comprehensive profile of the normal mouse WAT proteome.The WAT proteins were digested and then peptides were analyzed by label-free 2DLC/MS/MS approach.The resulting data was used to produce a comprehensive map of the mouse WAT proteome.Meanwhile,we sought to provide insight into WAT function based on protein functional annotation,and predict potentially secreted proteins.Results:A total of 6,039 proteins in WAT were identified,among which 5,160 were quantified(spanning a magnitude of 106)using an intensity-based absolute quantification algorithm,and 3,117 proteins were reported by proteomics technology for the first time in WAT.To comprehensively analyze the function of WAT,the proteins were divided into three quantiles based on abundance and we found that proteins of different abundance performed different functions.High-abundance proteins(the top 90%,1,219 proteins)were involved in energy metabolism;middle-abundance proteins(90-99%,2,273 proteins)were involved in the regulation of protein synthesis;and low-abundance proteins(99-100%,1,668 proteins)were associated with lipid metabolism and WAT beiging.Furthermore,800 proteins were predicted by SignalP4.0 to have signal peptides,265 proteins had never been reported,and five have been reported as adipokines.The above results provide a large dataset of the normal mouse WAT proteome,which might be useful for WAT function research.