Comparison Of Active Ingredients Of Banlangen Decoction Pieces And Banlangen Formula Granules

Radix is the dry root of the crucifer plant Isatis indigotica Fort.It was first recorded in the "Shen Nong's Herbal Classic" and is mainly produaced in Hebei,Anhuii,Jiangsu,Henane and other provinces.Sexual bitterness,cold,heart,stomach,has the effect of clearing away heat and detoxifying,cooling blood and pharynx,used for warming disease,spotted purple,dark purple tongue,phlegm,throat,rotten throat,big head lice,erysipelas,cockroach It is swollen and is often used clinically for viral diseases and bacterial infections.Objective: Through qualitative identification of Banlangen decoction pieces and Banlangen formula granules,this paper reduces or eliminates the patients' doubts about the new dosage form of Banlangen formula granules,and quantifies the main active ingredients of Banlangen Decoction Pieces and Banlangen granules.The input amount of the raw material medicine is converted into the minimum packaging specification of the traditional Chinese medicine formula granule according to the process parameters,and the clinical medication is better guided.Experimental method: The main active ingredients of Radix isatidis and Radix isatidis granules were extracted by water extraction and alcohol precipitation method.Qualitative study was carried out on the granules of Banlangen and Banlangen by thin-layer chromateography.And the high-performance is liquid chromateography was used to establish the granules of radix isatidis.Uridine,guanosine,adenosine,and the method for determining the content of Yichun.Experimental results: take 3g of medicinal material powder,accurately weighed,add 100 m L of water to boil 2 times,the time is 2h,lh,filtered,the filtrate is combined to 10 m L,add absolute ethanol to make the alcohol concentration reach 65%,4 ° C static Set for 24 h,filter,recover ethanol,transfer the concentrate to a 10 mL volumetric flask,add water to volume,shake well,that is the extract.The membrane was filtered through a microporous membrane(0.45 ?m),and the filtrate was taken as a test solution.The adenosine and argininecomponents were identified by thin-layer chromatography to identify the granules and arginine components.The uridine,guanosine and adenosine in Banlangen decoction were identified by high performance liquid chromatography.Significantly higher than the Banlangen formula granules of the other two manufacturers,the amount of sapphire formula particles used in clinical use should be a multiple of the minimum package size.