Effects Of Qinbo Decoction Combined With Mesalazine On Serum Inflammatory Factors In Rats With Ulcerative Colitis

Objective: To compare the expression levels of TGF-?1,IL-6 and IL-17 in serum of rats with ulcerative colitis(UC)before and after intervention with Qinbai Decoction and mesalazine,and to explore the therapeutic effect and influencing mechanism of Qinbai decoction,so as to provide a basis for its clinical application in the treatment of UC and explore new therapeutic methods and ideas.Methods:In order to construct a cell-mediated immunoreactive UC rat model,the method of TNBS + ethanol induction was used.Ten randomly selected non-model rats were divided into normal control group(NC).The successful rats were randomly and equally divided into model group(UC),Qinbai Decoction group(QB),mesalazine group(MS)and Qinbai decoction combined with mesalazine group(M+Q).After 3weeks of retention enema treatment,the serum levels of TGF-?1,IL-6 and IL-17 were measured by ELISA.The general condition,weight change and colon tissue injury of rats in each group were observed and recorded,and the disease activity index(DAI)and the degree of colon tissue injury of rats in each group were scored.Results: Compared with the normal control group,the general condition and body weight of the model group rats were significantly lower than those of the normal control group,and the degree of colon tissue injury was significantly higher than that of the normal group.The serum levels of TGF-?1 in the model group rats were decreased,while the levels of IL-6 and IL-17 were significantly increased.Compared with the model group,the levels of TGF-?1,IL-6 and IL-17 in the model group rats were significantly increased.The expression levels of IL-6 and IL-17 in serum of rats in the decoction combined with mesalazine group were down-regulated with statistical significance(p < 0.05).The expression levels of IL-6 and IL-17 in serum of rats in the Qinbai decoction combined with mesalazine group were down-regulated with statistical significance(p < 0.01).Compared with the Qinbai Decoction group,the levels of IL-6 and IL-17 in serum of rats in the Qinbai decoction combined with mesalazine group were down-regulated with statistical significance(p < 0.01).The expression of IL-6 in serum of rats in Qianbai Decoction combined with Mesalazine group had no statistical significance(p > 0.05),but the expression of IL-6 in serum of rats in Qianbai Decoction combined with Mesalazine group had statistical significance(p < 0.05).Compared with model group,the expression of IL-6 in Qianbai Decoction group,Mesalazine group,Qianbai Decoction combined with Mesalazine group had statistical significance(p < 0.05).The expression level of serum TGF-?1 was up-regulated,with significant difference(p < 0.05).The expression level of serum TGF-?1 was significantly up-regulated in the Qinbai decoction combined with mesalazine group(p < 0.01).Compared with the Qinbai Decoction group and the mesalazine group,the expression level of serum TGF-?1 in the Qinbai decoction combined with mesalazine group was up-regulated,with significant difference(p < 0.05).The general condition,weight change and the degree of colon tissue injury in Qinbai Decoction group,Mesalazine group and Qianbai decoction combined with Mesalazine group were improved in varying degrees compared with model group.Conclusion: Qinbai decoction combined with mesalazine can improve the general condition of UC rats,promote weight recovery,repair ulcerative colitis mucosal tissue and reduce pathological scores;the therapeutic effect of Qinbai decoction combined with mesalazine on ulcerative colitis may be through inhibiting the expression of IL-6and IL-17,promoting the expression of TGF-?1,to improve the pathological changes of ulcerative colitis.