Beet Protein Kinase And Phosphatase Superfamily Analysis And Interaction Network Construction

Environment is an important factor affecting plant growth and morphogenesis.Plants are always stimulated by various external stimuli during their growth.Signal transduction is the most important step for plant cells to respond to environmental stimuli,and protein phosphorylation is a key step in signal transduction.Therefore,it is of great significance to identify the protein kinase and protein phosphatase families of sugar beet and construct the interaction network of protein kinase and phosphatase related to abiotic stress response for understanding the mechanism of stress response of sugar beet and cultivating more stress-tolerant varieties.In this study,protein kinases and phosphatases in sugar beet were identified.752 protein kinases and 143 protein phosphatases were identified in sugar beets.Protein kinases belong to 9 superfamily,and protein phosphatases belong to 7 superfamily.Subsequently,the three-dimensional structures of all candidate protein kinases and protein phosphatases in sugar beet were predicted.Based on the three-dimensional structure of protein kinase and phosphatase,the interaction networks of protein kinase and phosphatase in endoplasmic reticulum,mitochondria,plastids,nucleus,cytoplasm and membrane were constructed.7 pairs of protein interactions were identified in endoplasmic reticulum,247 pairs in mitochondria,49 pairs in cytoplasm,715 pairs in nucleus,and 2992 pairs in cytoplasm and membrane proteins.In this study,133 transcriptome data of sugar beet under abiotic stress were collected from the SRA database,and the transcriptomes of sugar beet leaves under short-term and long-term lowtemperature stresses lacking in the database were sequenced.Subsequently,the above transcriptome data were integrated to construct a co-expression network related to the response of sugarbeet to abiotic stress.Combined with the interaction network and coexpression network of betaine protein kinase and phosphatase protein,we screened the interaction relationship pairs that simultaneously meet the protein spatial structure interaction relationship,located in the same organelle and adjacent organelle membrane,and have co-expression pattern.In this study,a total of 55 genes encoding protein kinases and phosphatases were found to be up-regulated in all low-temperature stress experiments,among which 43 protein kinases and phosphatases constituted a low-temperature response network with 37 pairs of interactions.Roots in 38 and 27 respectively encoded protein kinase and phosphatase gene in all cryogenic stress experiments are raised or lowered expression,63 of the low temperature response of the encoding protein kinase and phosphatase contains 54 of interaction relationship between low temperature response network,including a total of 28 protein function annotation display related to low temperature stress.