Potential Application Of In Situ Synthesis Of Nanozymes Based On Foamed Graphene As Electrodes

With the rapid development of the global economy,the energy shortage and environmental pollution caused by the consumption of traditional fossil fuels are increasing.In the face of this severe challenge,increasing the development of renewable energy is of great significance to ensuring energy security and maintaining sustainable economic development.EBFC is a green energy conversion device to convert the chemical energy of biomass into electric energy.It has the advantages of good biocompatibility and high output power.One of the significant defects of such cells is the low charge transfer efficiency between enzymes and electrodes.In this paper,we synthesized nanoenzymes in situ on three-dimensional foam graphene with porous structure to build a new enzyme biofuel cell and self-energy biosensor,and carry out the research work on biomass chemical energy and electric energy conversion and utilization.On the one hand,from the perspective of nanomaterials design and synthesis,three nanoenzymes in situ ISLM(in situ synthesis laccase ISAu and peroxidase in situ synthesis ISPd Fe)were successfully synthesized and applied as biological electrodes in enzymatic biofuel cells.On the other hand,thanks to the perspective of the efficiency improvement of the nanoenzymes,the nanomaterials synthesized in this experiment have good stability,enzyme activity and electrochemical activity,which have the potential to build fuel cells.The main research content includes the following two aspects:(1)A dual-nanozyme self-powered biosensor based on the in-situ synthesis of laccase-like ISLM and glucose-like oxidase ISAu on foamed graphene was constructed,which can realize the detection of 2,4-dichlorophenol(2,4-DP).The in situ synthetic laccase-like ISLM was synthesized by a one-step method.More laccase-like enzymes are supported by the foamed graphene with a larger specific surface area,and a larger electrochemically active area is provided.This kind of laccase can not only achieve color development within 15 minutes,but also suitable for a wide p H range,besides,it has good affinity.The in situ synthetic laccase-like enzyme ISAu was also synthesized by one-step method to build the dual-nanozyme self-powered biosensor.The self-powered biosensor can realize quantitative detection of phenolic substances.Therefore,the self-powered biosensor proposed in this work provides a new avenue for the detection and elimination of phenolics.(2)A nano-enzyme biosensor based on the in situ synthesis of peroxidase-like on foamed graphene was established,realizing the quantitative and sensitive detection of peroxide H₂O₂.In this experiment,a one-step hydrothermal method was used to synthesize in-situ peroxidase-like ISPd Fe nanomaterials.This material is sensitive to p H,has a large electrochemically active area,and can achieve color development within two hours.The detection and elimination of oxides provides a new approach.In conclusion,this work provides a new idea for dual nanozymes to replace the cathode and anode of enzymatic biofuel cells to increase the application range.