Fluorescence-enhanced Probes Are Used In The Detection And Research Of Glutathione

The qualitative and quantitative detection of specific analytes is very important in the fields of life sciences and environmental monitoring.Glutathione（GSH）,as a biological thiol tripeptide compound containing active sulfhydryl groups in the organism,participates in many important physiological processes in the organism,such as regulating oxidative stress in the organism,participating in some biological signal transduction,and It can chelate with metal ions as an antidote;as a functional biomolecule,research on GSH is gradually deepening.Reported in relevant literature,it has been found that GSH can be used as a physiological indicator of many diseases,such as diabetes and Alzheimer’s disease,Parkinson’s disease and some cardiovascular diseases,so the development of rapid and accurate methods for detecting GSH has very important medical value.Fluorescence methods have received widespread attention because of their high sensitivity,fast response,easy operation,and low cost.With the in-depth study of fluorescence analysis methods,there are also higher requirements for the detection of targets by fluorescence methods.Based on a large number of literature investigations,we have designed the Turn on response type to improve the sensitivity of detection and reduce background interference.The fluorescent probe is used for the detection of GSH;this article aims to achieve high-efficiency,visualization and accurate detection of GSH,combining the advantages of fluorescent nanomaterials and organic fluorescent dye molecules in fluorescence analysis methods,respectively designing and synthesizing nano-fluorescence and organic fluorescence turn on-responsive signal probes.For GSH testing,the specific content is expanded as follows:（1）The electrostatic interaction between the gold nanoclusters（Au NCs）and the aminated mesoporous silica spheres（MSN）makes the Au NCs gather in the pores and surface of the MSN,and obtain an enhanced stability and fluorescence signal enlarged nanocomposite gold nanocluster-silica spheres（MSN-Au NCs）.Based on the internal rate effect（IFE）,the fluorescence of MSN-Au NCs at 570 nm is absorbed at 330-470nm and 500-570 nm for oxidized 3,3’,5,5’-tetramethylbenzidine（ox TMB）.When there is a response target GSH,reducing ox TMB becomes TMB and blocks the internal rate effect between ox TMB and MSN-Au NCs,causing the fluorescence of the system to gradually recover.The Turn on responsive fluorescent probe designed in this experiment can not only reduce background interference,but also use MSN to construct a signal amplification strategy to improve the sensitivity of detection.The fluorescence detection of GSH has obtained a good linear response,and the lowest detection limit is 0.12μM.We successfully applied the probe to serum detection and obtained a good recovery rate.（2）The reaction of rhodamine hydrazide with 3,5-dibromo-4-hydroxy benzaldehyde yields a single-peak emission probe L,which can be used for the dual-signal detection of Cu²⁺and GSH with fluorescence and Colorimetric under the light synergistic effect.First,after adding Cu²⁺to the buffer solution of the probe under light conditions,the Cu²⁺and the probe undergo ring-opening coordination under the light-induced effect,and the solution gradually changes from colorless to pink with a clear UV absorption peak appear at 560 nm.The spectrometer showed that the probe changed from the original single peak to double peaks at 525 nm and592 nm,and the fluorescence intensity of the double peak decreased linearly with the increase of Cu²⁺concentration;when GSH was added again,GSH chelated with Cu²⁺to reduce Cu²⁺Under drag,the double peaks gradually increased to become single peaks and the color gradually faded.We chose to quantify the response concentration of Cu²⁺and GSH with the fluorescence intensity at 525 nm,and the detection limits were 0.36μM and 3.45μM,respectively;in addition,the UV generated by the probe The signal also has a linear response to Cu²⁺and GSH,and the detection limits are0.54μM and 0.28μM,respectively.The probe also showed very good selectivity for the target,and a good recovery rate was also obtained in the actual serum sample.