| Background:Mild cognitive impairment（MCI）and Alzheimer’s disease（AD）are recognized risk factors for middle-aged and elderly people.Aerobic training（AT）can improve the ability to work memory and inhibitory control of healthy elderly.Flexi-Bar vibration exercise,as a special form of exercise with aerobic energy as the energy supply and a vibration exercise as the exercise mode,has been confirmed to have a positive effect on the cognition of the middle-aged and elderly.This study aimed to evaluate whether Flexi-Bar exercise can be used as an effective intervention to delay the decline of cognitive function caused by aging and its potential mechanism.Methods:Thirty middle-aged and elderly sedentary women aged 45-65 were recruited from Guangzhou Cadre Sanatorium and randomly divided into an intervention group（N=15）and a control group（N=15）.The exercise program of the intervention group was as follows: 1 hour every Monday,Wednesday,and Friday at an intensity of 50%to 65% of the maximum heart rate,Flexi-Bar exercise intervention for 8 weeks,and real-time heart rate monitoring during the intervention.The control group maintained normal living habits.Before and after the intervention,the two groups of subjects were tested for related functions and the samples were collected:（1）The reaction time and accuracy rate of the subjects were collected while performing the Stroop test,and at the same time,the NIRX near-infrared functional brain imaging system was used to collect the tasks Hemodynamic changes in the prefrontal lobe under two conditions.（2）Enzyme-linked immunosorbent assay was used to quantitatively detect the concentrations of BDNF,TNF-α and Irisin in the serum of the two groups.（3）Illumina sequencing was used to perform small RNA sequencing on the serum of the subjects.Results:（1）Compared with the control group,there was no significant difference between the groups in response time and correct rate（P>0.05）,but there was a significant difference in response time within the intervention group（P<0.05）;（2）Compared with the control group,the content of oxygenated hemoglobin in the dorsolateral prefrontal lobe of the brain increased significantly after the exercise intervention（P<0.05）;（3）the BDNF concentration in the intervention group increased significantly after the exercise intervention（P<0.05）.There was no difference between groups（P>0.05）.Compared with the control group,the concentration of inflammatory factor TNF-α was significantly different between groups（P<0.05）,As for the Irisin,we just found the increase after the intervention in the FE group;（4）Through the analysis of the sequencing results and the results of previous studies,we found that among the 48 mi RNA with differences between groups,10 mi RNA such as mi R-425-5p,mi R-425-3p,and mi R-29c-5p,which were highly expressed in AD patients,were significantly down-regulated after the intervention.However,the expression of four mi RNA,such as mi R-139-3p and mi R-181-5p,which were low expressed in AD patients,was significantly up-regulated after the intervention.Conclusion:Flexi-bar exercise intervention can improve the function of inhibitory control in middle-aged and elderly women,which is mainly reflected by lower reaction time in the Stroop task and higher blood oxygen content in the dorsolateral prefrontal cortex.Meanwhile,higher levels of BDNF and lower levels of TNF-α also predicted increased synaptic regeneration and decreased brain inflammation.The highly expressed mi RNAs in AD patients are all down-regulated to varying degrees after the intervention,which may affect the expression of mi RNAs affecting cognitive function. |
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