Research On The Phenomenon Of Decline In The Solubility Of Silk Protein In Silk Cultural Relics And Its Solution

Silk is a textile made of silk,man-made fibers and synthetic fibers.According to textual research,the history of sericulture and silk reeling in China can probably be traced back to the Yin and Shang dynasties,and silk has been used in all dynasties.From the beginning of ghosts and gods,as sacrificial clothing to daily life clothing,it runs through the entire history of Chinese civilization.The study of silk cultural relics plays an important role in the study of social politics,economy,and culture at that time.At the same time,silk weaving skills are contemporary intangible cultural heritage,and the study of silk cultural relics plays an important role in promoting traditional Chinese culture and enhancing cultural self-confidence.As a natural polymer protein,silk is easily affected by the burial environment and preservation environment,and degrades and changes the structure.Therefore,the preservation of a large number of silk cultural relics is not good.In order to better formulate scientific and effective protection methods for silk cultural relics,modern analytical techniques for the morphology,performance,chemical properties,molecular structure and other characteristics of silk raw materials have been widely used in the analysis and testing of silk cultural relics.The structural analysis of silk protein is the basis for studying the aging characteristics of silk fiber.At present,a large number of relevant analysis and detection techniques need to dissolve silk protein.However,the existing silk protein dissolving system has a decline in solubility for severely aging silk samples,so it is improved.The dissolution efficiency of silk cultural relics has become a key issue in the protection of silk cultural relics.Silk protein is the main component of silk cultural relics.This article takes silk protein in silk cultural relics as the research object to discuss how to improve the dissolution efficiency of silk protein in aging silk samples.The main content of this article includes:a.Select representative environmental factors to prepare silk samples in different aging states;b.Observe and record the dissolution status of silk samples in different aging states,and initially explain the reasons for the decline in the dissolution rate of silk cultural relics;c.On the basis of the existing dissolution methods,the method to improve the dissolution rate of silk protein in the aged silk cultural relics was explored by optimizing the dissolution conditions.Silk cultural relics are scarce and precious and cannot be used in large-scale experimental research.This study selected the most commonly used methods of thermal oxidative aging and hydrolytic aging in the current study of silk aging characteristics to simulate the deterioration state of silk cultural relics and prepare sufficient samples with different degrees of aging.And record the aging characteristics of each simulated sample through direct observation,color analysis and mechanical strength analysis difference analysis.The current mainstream protein dissolving system-calcium alcohol dissolving system is used to dissolve samples with different aging degrees and observe the dissolution situation.The experimental results show that as the degree of sample aging deepens,the dissolution efficiency of the sample continues to decrease,and it is even difficult to completely dissolve the sample with severe aging.In response to this phenomenon,SDS-PAGE gel electrophoresis analysis,infrared spectroscopy analysis,and scanning electron microscopy analysis were performed on the simulated aging samples.The results showed that as the aging degree of silk increased,the silk fiber was broken;the molecular weight of extractable protein decreased;the molecular structure of the protein changed,and the ratio of crystalline areas in the protein conception was increasing.It is generally believed that the aging degradation of silk starts from the amorphous region.The α-helix and random coil structure in the amorphous region are explained,the content decreases,and the β-sheet structure content in the crystalline region increases.The deeper the aging degree of silk,the larger the proportion of crystalline area.Therefore,the molecular structure change of silk is one of the reasons that affect the solubility of silk protein.The extraction method of silk protein from aged silk samples was optimized by controlling the two variables of dissolution temperature and dissolution time.A modern silk sample without any treatment was used as a standard sample,and a standard protein curve was drawn using the Bradford detection method.It was found that the concentration of solubilized silk protein was positively correlated with the spectrophotometric value of the sample at A595nm.Choose two representative samples aged at 150℃ for 48h and aging at 230℃ for 5h,choose the three dissolution temperatures of 80℃,90℃,100℃ and the dissolution time visible to the naked eye,2h,24h three dissolution times,through the control variables,exhaustive For all the dissolution conditions,the dissolved solution was subjected to dialysis and ultrafiltration,and the concentrated solution was tested by Bradford detection method.After integrating the standard protein curve,it was concluded that the dissolution temperature of the lighter aging sample was 80℃,and the dissolution time was 80℃.The time when the dissolution is completed is visible to the naked eye;the dissolution temperature for the best dissolution conditions for the heavier aging samples is 100℃,and the dissolution time is 24h.