Clinical Study On The Correlation Between Inflammatory Cytokine Levels And Immune Function In Patients With Sepsis

Background Sepsis has always been one of the main causes of death in intensive care units,and its definition has been changing.From sepsis1.0 to sepsis3.0,the understanding of sepsis has been updated,and people’s understanding of the pathophysiological mechanism of sepsis The understanding is also gradually deepening,from the systemic inflammatory response syndrome（SIRS）,which was initially thought to be caused by excessive inflammatory response,to the current belief that the mechanism of sepsis is no longer only composed of inflammatory response,but also the immune suppression of the body.After continuous in-depth research in the clinical diagnosis,treatment,and immune pathophysiology of sepsis,despite continuous improvements in antibiotic management,fluid resuscitation,and ventilator use,sepsis is still the death of patients in the intensive care unit（ICU）.one of the main reasons.We believe that early and accurate identification of patients,predicting disease progression and prognosis,improving the timeliness of treatment,and improving patient survival are the keys to sepsis treatment.At present,many relevant studies have suggested that inflammatory factors are related to the immune function of sepsis,but there are few studies on the relationship between the level of inflammation and immune function in patients with sepsis.The relationship between inflammatory cytokines and immune function indicators was discussed.The inflammatory factors in this experiment were represented by IL-2,IL-4 and IL-10,and the immune function was represented by CD4,CD8 and CD4/CD.Objectives To compare the relationship between inflammatory factors in patients with sepsis andpatients with septic shock,to explore the relationship between the levels of interleukin （IL）-2,IL-4,and IL-10 in patients with different stages of sepsis,and to further study inflammation level and immune function.Methods A total of 98 patients with sepsis admitted to Zhengzhou People’s Hospital from January 2020 to December 2021 were selected.After screening,57 patients were finally enrolled.According to the diagnosis at the time of admission,they were divided into a sepsis group and a septic shock group.The general information of the patients was collected on the day of admission,and venous blood samples were collected to measure IL-2,IL-4,and IL-10 by enzyme-linked immunoassay kit.Quantitative analysis was performed,and the 28-day prognosis of patients was followed up,and death was used as a poor prognostic indicator.To analyze the levels of inflammatory factors and immune indicators in different sepsis processes,to explore the correlation between inflammatory factors and immune function,and to explore their relationship with prognosis.Results 1.The levels of IL-2,IL-4,and IL-10 in the septic shock group were higher than those in the sepsis group,while the levels of CD4,CD8,and CD4/CD8 were significantly lower than those in the sepsis group,and the difference between theg roups was statistically significant.Significance（P<0.05）.2.IL-2,IL-4,IL-10 in sepsis patients were negatively correlated with CD4 and CD8（P<0.05）,but had no correlation with CD4/CD8（P>0.05）.3.ROC curve analysis of the prediction results of IL-2,IL-4,IL-10 levels on the poor prognosis of patients with sepsis,AUC values were 0.744,0.698,0.871.Conclusions The level of inflammation in patients with sepsis increased with the aggravation of the disease,and the levels of inflammatory factors IL-2,IL-4,and IL-10 were negatively correlated with immune function indicators CD4 and CD8.At the same time,the elevated levels of inflammatory factors IL-2,IL-4,and IL-10 are associated with poor prognosis in patients with sepsis.