Physicochemical Characterization And Degradation Products Osteogenic Activity Of Three Collagen Membranes

Objective:The aim of the present study was to evaluate the physicochemical properties of three kinds of collagen membranes from different sources,by evaluating surface topography,porosity,mechanical properties,degradability,contact angle and the effects of their degradation products on bone marrow mesenchymal stem cells.The properties of collagen membranes help to provide a basis for the selection of clinical cases.Methods: The surface topography of three kinds of collagen membranes was observed by scanning electron microscope,such as roughness and fiber arrangement;.The three collagen membranes were soaked in PBS solution to measure their porosity or swelling properties.The tensile strength and elongation at break of collagen membranes were measured by universal testing machine.The collagen membranes were degraded in PBS solution,artificial saliva,and collagenase solution,and the degradation rate was measured after 1,2,3,7,14,and 21 days,and the microscopic surface of the collagen membranes was observed by scanning electron microscope after degradation.The contact angle of the three collagen membranes was measured by contact angle measuring instrument.The effects of collagen membrane degradation products on the cell proliferation and cell differentiation of BMSCs were compared by cell experiments.Results: The smooth surface and rough surface of different collagen films were observed by scanning electron microscope.No matter low or high multiple observation,the surface micro-morphology of Jayyalife dermis and Bio-Gide is complex,and there are a large number of collagen fibers arranged in disorder,while the smooth surface of Jayyalife dermis and Jayyalife is relatively dense.From the low multiple observation,there is no significant difference between the rough surface and the smooth surface of Giteri,while from the high multiple observation,a small amount of collagen fibers can be seen on the rough surface.In the cross-sectional observation,Jayyalife dermis and Bio-Gide showed obvious delamination and micropore-like structure,while the delamination of Giteri menbrane was not obvious and micropore-like structure.The porosity of Bio-Gide is the largest,Giteri is the second,and Giteri is the smalles.Under dry condition,the tensile strength of Jayyalife dermis and Giteri is larger and there is no significant difference,and elongation at break of Bio-Gide is the largest.While in wet state,the tensile strength of three kinds of films decreases obviously,and the tensile strength of Bio-Gide is the smallest,and the tensile strength of Jayyalife dermis is the largest;in elongation at break,Jayyalife dermis and Bio-Gide increase obviously.Compared with the three degradation solutions,the degradability of collagenase solution was the highest.The degradation rate of Giteri was the highest in PBS solution.The degradation rate of Bio-Gide and Giteri was the highest under artificial saliva and collagenase degradation,and the degradation of Jayyalife dermis was the smallest.Scanning electron microscope showed that the surface of collagen membrane was flat,and the collagen fibers disappeared and the roughness decreased.The contact angle of rough surface is smaller than that of smooth surface.The contact angle of Bio-Gide is smaller than that of Jayyalife dermis and Giteri,and its hydrophilicity is stronger.With the passage of time,the proliferation effect of each group on BMSCs cells gradually increased.The proliferation effect of Jayyalife dermis and Giteri degradation products on BMSCs cells was higher than that in Bio-Gide group.With the increase of time,the ALP activity increased gradually among the groups,and the ALP activity was the highest in the Giteri group on the 14 th day.Conclusion: In this study,through the exploration of surface micromorphology,porosity,mechanical properties,degradation characteristics,contact angle and osteogenic characteristics of degradation products,it is concluded that the surface morphology of Jieya dermis and Bio-Gide is more conducive to cell attachment and growth.The porosity of Bio-Gide is more contribute to the diffusion of nutrients.Jieya dermis and Gitri have higher mechanical strength,which is more suitable for cases with high soft tissue pressure.The degradation of Jieya dermis is the smallest,and it is more stable during bone regeneration.The hydrophilicity of Bio-Gide is stronger.Jieya dermis and Gitri degradation products have a greater influence on the proliferation and differentiation of BMSCs.Therefore,different collagen membranes have their own advantages,so we can choose the corresponding collagen membrane according to clinical needs.