| Objective:To study the chemical constituents and anti-inflammatory activity of V.auraria,so as to provide a high-quality lead compound for research and development of natural anti-inflammatory drugs,and to lay a foundation for the development and utilization of wasp resources.Methods:Petroleum ether,chloroform,ethyl acetate,n-butanol and water fractions were obtained from the ethanol extract of V.auraria by extracting with different polar solvents.The lipid soluble component of petroleum ether fraction was analyzed by GC-MS.Other fractions were separated and prepared by systematic separation method,while the structures of compounds were characterized and analyzed by several modern spectral techniques.The dopamine and its derivatives isolated and identified from V.auraria were observed for effects on the expression of tumor necrosis factor-α（TNF-α）with mouse macrophage RAW264.7 induced by lipopolysaccharide（LPS）and interferon-γ（IFN-γ）as the inflammatory model.Results:A total of 23 compounds were identified from petroleum ether fraction of the extract from V.auraria,and their principal components were Ethyl oleate（33.72%）,Methyl elaidate（20.40%）,Cholesterol（5.00%）.A total of 33 compounds were isolated from chloroform and ethyl acetate fractions and identified as 2’,3’-dihydroxypropyl 2-hydroxy-4-methylpentanoate（1）,trans-2-（3’,5’-dihydroxyphenyl）-3-acetylamino-6-（2-hydroxyacetyl）-1,4-benzodioxane（2）,trans-2-（3’,4’-dihydroxyphenyl）-3-acetylamino-6-carboxy-1,4-benzodioxane（3）,plancyamide B（4）,N-acetyl-dopamine（5）,trans-2-（3’,4’-dihydroxyphenyl）-3-acetylamino-7-（N-acetyl-2’’-aminoethyl）-1,4-benzodioxane（6）,trans-2-（3’,4’-dihydroxyphenyl）-3-acetylamino-6-（N-acetyl-2’’-aminoethyl）-1,4-benzodioxane（7）,trans-2-（3’,4’-dihydroxyphenyl）-3-acetylamino-7-（N-acetyl-2’’-aminethylene）-1,4-benz odioxane（8）,（2R,3R）-2-（3’,4’-dihydroxyphenyl）-3-acetylamino-7-（N-acetyl-2’’-aminoethyl）-1,4-benzodioxane（9）,divesamides A（10）,Cyclo（Pro-Thr）（11）,Cyclo-（Ala-Pro）（12）,Cyclo（Gly-Pro）（13）,Cyclo-（Pro-Tyr）（14）,Cyclo（Pro-Pro）（15）,N-4-hydroxyphenylethanyl acetamide（16）,Catechol（17）,4-Hydroxybenzaldehyde（18）,methyl 3-（4-hydroxyphenyl）propionate（19）,2-（4-hydroxyphenyl）ethyl acetate（20）,3,4-dihydroxy-phenethyl alcohol（21）,3,4-dihydroxyphenyl ethanol ketone（22）,Protocatechuic acid（23）,protocatechuic acid methyl ester（24）,N-Acetyl isoleucine（25）,1H-Indole-3-acetic acid（26）,1-Methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid（27）,1-methoxy-1-oxopropan-2-yl 2-hydroxypropanoate（28）,4-Methoxybenzyl alcohol（29）,Papuline（30）,2-（4-Methylphenyl）ethanol（31）,Thymine（32）,α-ethyl glucoside（33）.While in the compounds,1-3 were new compounds and 4-33 were the compounds isolated from V.auraria for the first time.At a concentration of 300 μg/m L for administration,the ethanol extract,water extract,ethyl acetate fraction and n-butanol fraction inhibited the expression of i NOS m RNA in RAW264.7 cells induced by LPS+IFN-γ without significant toxicity to RAW264.7 cells,while at 30 μg/m L,compounds 4,8 and 9 inhibited the expression of TNF-α m RNA in RAW264.7 cells induced by LPS+IFN-γ without significant toxicity to RAW264.7 cells.Conclusion:The compounds identified from V.auraria are various.Specifically,the petroleum ether fraction contains phenols,alcohols,esters,acids and aldehydes,while the chloroform and ethyl acetate fractions contain dopamine and its derivatives,phenols and cyclic peptides.The ethyl acetate and n-butanol fractions are critical for the anti-inflammatory activity of V.auraria.Compounds 4,8 and 9 have a potential anti-inflammatory activity. |
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