Diammonium Glycyrrhizinate Improves Liver Injury In Severely Scalded Rats By Alleviating Endoplasmic Reticulum Stress

Background The risk of death from severe burns has always been a major medical problem to be solved,and liver dysfunction after severe burns is one of the early risk elements that will impact the prognosis of patients.However,the potential mechanism of liver injury due to severe burns remains unclear.Glycyrrhizic acid is an active ingredient extracted from the natural herb-licorice that has a protective effect on the liver,and diammonium glycyrrhizinate（DG）,that is a third-generation glycyrrhizic acid preparation,is often used as a drug to protect the liver in the clinic.However,whether it can improve liver damage caused by severe burns is unclear.Therefore,this research focuses on the protective function of DG on liver injury after severe burns and its implicit mechanism.Methods Take 54 female SD rats of weight about 200-250 g,and distribute them into sham injury group,simple burn group,burn + diammonium glycyrrhizinate group,and each group is divided into three time points of 24 h,48h and 72 h,and 6 rats in each phase points of each group are in each group.Rats in the sham injury group caused 30% TBSA simulated injury,and simple burn group and burn+ diammonium glycyrrhizinate group caused 30%TBSA three degree burns.Liquid resuscitation was carried out immediately after injury in the simple burn group and the burn+ diammonium glycyrrhizinate group;24h phase point rats in the scald + diammonium glycyrrhizinate group were injected with diammonium glycyrrhizinate solution once through the abdominal cavity at 1 h after injury;48h phase point rats in the scald + diammonium glycyrrhizinate group were injected with diammonium glycyrrhizinate solution once through the abdominal cavity at1 h,25 h and 49 h after injury;Each group of rats with each time phase point was given abdominal aortic blood,bloodletting and liver tissue at the corresponding time phase point（24h,48 h,72h）.Arterial blood centrifugation,and through blood biochemical analyzer,serum to detect the levels of aspartate aminotransferase alanine aminotransferase and lactate dehydrogenase.Enzyme-linked immunosorbent assay to inspect ornithine carbamoyltransferase levels of serum;the Real-time fluorescence quantitative reverse transcription PCR was used to inspect the m RNA levels of Caspase-3,GRP78 and PERK.Through Western blotting to inspect the protein levels of Caspase-3,GRP78 and PERK.For One-way ANOVA analysis of data rows,multiple comparisons were analyzed by using the Bonferroni test.Results1.The liver dysfunction indicators of the simple burn group at each time point were obviously increased,and the levels of TP and Alb were lower than the sham injury group;contrast with the simple burn group,the indicators of liver dysfunction in the burn + DG group reduced significantly,and the levels of total protein and albumin raised;2.72 h after injury,the serum OCT of the simple burn group was elevated,and the OCT of the burn + DG group decreased compared with the simple burn group;3.72 h after injury,contrast with the sham injury group,Contrast with sham injury group,the expression of apoptosis related protein in the simple burn group was increased;And the expression of apoptosis related proteins in the burn + DG group was decrease.4.72 h after injury,Contrast with the sham injury group,the expression of ERS related protein in the simple burn group was increased;And the expression of ERS proteins in the burn + DG group was decrease.5.72 h after injury,the hepatic tissue of rats in the sham injury group was normal,the hepatocyte morphology was normal,the array of hepatocyte was regular,and no apparently inflammatory cells was seen;the hepatocyte arrangement of rats in the simple burn group was disordered,and obvious cytoplasmic looseness was visible,accompanied by diffuse fat lesions and moderate inflammatory cell infiltration;rat hepatocyte in the burn +DG group were arranged relatively regularly,and scattered steatosis was visible,accompanied by a small amount of infiltration of inflammatory cells.Conclusion1.After severe burns,the hepar function of rats is apparently damage;the use of diammonium glycyrrhizinate can improve the liver function damage of rats,and the longer the use time,the better the effect;2.After severe burns,the liver tissue and cells of rats were damaged;after repeated use of diammonium glycyrrhizinate,the damage to liver tissue and cells was lighter than that of the simple burn group;3.After severe burns,liver apoptosis increases;and DG can reduce the apoptosis reaction of the liver after burns;4.Severe burns can induce the occurrence of endoplasmic reticulum stress in the liver,while diammonium glycyrrhizinate can inhibit this reaction,thereby effectively reducing liver damage.