P300 Response And Its Regulatory Mechanism In Medial Prefrontal Cortex Of Mouse

P300（P3）is a useful tool in both medical and engineering field,however,the mechanism it generates from cognizance and the neurochemical substrates remain imprecisely known.In this study,the influence of exogenous stimuli and endogenous decision behaviors on P3 was obtained.Event-related potentials（ERPs）were recorded from the medial prefrontal cortex（m PFC）of pre-trained awake mice in oddball paradigm,a significant larger P3 peek was observed in trials those low-probability target stimulus presented or with the licking gesture of mice.It may suggest that the presentation of stimulus and the decision of mice have influence on P3 generation.In order to investigate the mechanism,the event-related oscillations（EROs）of these data were analyzed.Gamma activity increased after the stimulus event,which may indicate that the stimulus event induced the enhanced local cell discharge activity.The phase amplitude coupling in the event-related Theta-Gamma band was found to be stronger in the low-probability presentation of the target sound stimulus or the mouse licking response,which may indicate that the presentation of the stimulus and the mouse decision have an effect on the synchronization of cellular activities.And the greater synchrony of cellular activity resulted in more significant P3 activity.The other purpose of this study is to explore the role of γ-aminobutyric acid（GABA）in formation of P3 component.Enhanced P3 amplitude was detected along with stronger event-related GABA release（ERGR）,after mice were stimulated by target tones or making the response decision.The result shows that P3 works related to the cognitive process and GABA engaged in that process.