Study On The Mechanical Properties Of Prostate Cancer By AFM

Biomechanical properties are closely related to the occurrence and progress of tumors.Mechanical forces participate in almost all levels of life activities,from molecules and cells to tissues and organs.Therefore,the study of tumor biomechanical properties can provide information on the mechanism of cancer progression,and may provide new methods for diagnosis,treatment and prognosis.In this study,we used Atmoic Force Microscopy to study the difference of tumor mechanical properties in the process of prostate cancer progression.Cluster analysis was used to evaluate the viscoelasticity of cellular and non cellular regions of tumor tissue to describe the changes of tissue mechanical properties caused by disease state.First,the viscoelasticity of prostate cancer cells were measured by atomic force microscopy at the single cell level,and the cell surface topography was obtained;Secondly,the nanomechanical properties of BPH and Prostate Cancer were compared by AFM,which the viscoelastic peak value of the woven sample decreases with the deepening of the malignant degree of the sample,and the distribution of the peak value becomes narrower by peak analysis.In addition,combined with ultrasound elastography,AFM has higher spatial resolution and sensitivity in the evaluation of tissue elasticity;Finally,cluster analysis algorithm is applied in dividing tissue viscoelasticity data to study the difference of mechanical properties between cellular and non cellular parts in prostate cancer.The results show that: the progress of prostate cancer will lead to the decrease of viscoelasticity in the tissue cell area,and slightly increase it in the non cell area.In conclusion,our results indicated that the nanomechanical properties of prostate tissue are directly related to the progress of cancer,which provides a new auxiliary method for the early diagnosis of prostate cancer in the future.