Development Of A Novel Quantitative Analysis Method For Intracellular Transport Of Nanoparticle

In recent years,the application of nanomaterials has been a novel research field in biology.Nanomaterials have been widely used in drug delivery,fluorescent probes and other biological fields,which play an extremely important role and have a wide range of research prospects.However,despite the great application prospect and scientific research value,people still lack an quantitative understanding of the movement mechanism and reaction principle of intracellular transport.In particular,there is a lack of quantitative understanding of the nanoparticles in intracellular transport.Although single particle tracking(SPT)and pair correlation function(p CF)have become important methods to study the intracellular transport process,each has its own obvious shortages.Single particle tracking is suitable for analyzing the long distance movement of a small number of nanoparticles in cells.Meanwhile,pair correlation function is suitable for analyzing the short distance movement of a large particle number in cells.Researchers need an analysis method that combines the two methods to make up for the shortcomings of the two methods.In this thesis,we established a new analysis method: i SPT-p CF-SDCM.This analysis method was mainly based on the scanning disk confocal microscope(SDCM),which combined two classical mathematical tool: single particle tracking(SPT)and pair correlation function(p CF).Our analysis method,for the first time,realized the combination of the two classical methods of single particle tracking and pair correlation function in one experimental platform.we also established a set of MATLAB based programs.The semi-automatic analysis of the analysis method also has been realized.On the other hand,we designed and constructed a nanoparticle intracellular transport model,an important method to study the interaction between various particles and structures of cells.We designed,built and implemented the model by using MATLAB.In this thesis,the first chapter is the introduction part;The second chapter discusses the process of a series of experiments to test the analysis method’s stability and reliability in aqueous phase and cellular environment;The third chapter discusses the process that we measured the kinetic characteristics of the movement in He La cells and BMSCs and the ability of nanoparticle passing through the barriers in He La cells and BMSCs by our analysis method;The fourth chapter discusses the construction and simulation of nanoparticle intracellular transport mathematical mode and showed the result of comparing the end of model and real data from cell experiment.