M2 Type Macrophage-derived Exosomal MicroRNA-23a-3p Promote OSCC Progression Through Down Regulate PTEN:A Primary Study

Background:More than 90% of oral cancers are squamous cell carcinomas.Oral squamous cell carcinoma(OSCC)is considered to be the most common maxillofacial and oral malignant tumors,with a high degree of malignancy.The current treatment method is mainly a combination of surgery and radiotherapy and chemotherapy,but the prognosis is still not ideal.Therefore,it is necessary to look for more malignant tumors.Effective treatment is necessary.In the tumor microenvironment,stromal cells release exosomes to participate in various signal transmissions between cells,and the exosomes of cancer cells or immune cells carry biological macromolecules or micro RNA(mi RNA)to regulate the tumor microenvironment and participate in the occurrence of tumors.development of.In this experiment,our research aims to explore the role of micro RNA-23a-3p in tumor-associated macrophages(TAM)exosomes in the progression of oral squamous cell carcinoma.Methods:First,through high-throughput sequencing to determine the expression differences of mi RNAs in the exosomes of various types of macrophages,find the differential mi RNAs highly expressed in M2 macrophages,predict their target genes through the mi RNA target gene database,and determine the mi R-23a-3p and PTEN are the research objects of this experiment.Then,CCK-8,RT-PCR,Western blot,and wound healing assay were used to detect the effects of mi R-23a-3p in M2 macrophage exosomes and M2 macrophage exosomes in vitro cell experiments on oral squamous cell carcinoma.The influence of cell line on proliferation,apoptosis,invasion and migration.Results: 1.The results of CCK-8,wound healing assay,PCR and WB proved that M2 type macrophages and their exosomes can promote the proliferation,invasion and migration of oral squamous cell carcinoma cells,and inhibit cell apoptosis;2.High-throughput sequencing results showed that mi R-23a-3p was highly expressed in M2 macrophages compared with M0 and M1 macrophage exosomes;3.CCK-8,wound healing assay,PCR and WB test results show that mi R-23a-3p in M2 type macrophage exosomes can promote the proliferation,invasion and migration of oral squamous cell carcinoma cells,and inhibit Apoptosis,thereby promoting the progress of OSCC;4.Target gene prediction,PCR and WB experiment results show that mi R-23a-3p in M2 macrophage exosomes targets PTEN and inhibits its expression.Conclusion:M2-type macrophages secrete exosomes and deliver mi R-23a-3p to oral squamous cell carcinoma cells through exosomes,and promote the invasion and migration of OSCC cells by down-regulating the expression of PTEN,and inhibit cell apoptosis.