Second-generation Sequencing Analysis Of Childhood Ph~+ And Ph-like Acute T Lymphocytic Leukemia

Objective:To screen the core genes of Philadelphia chromosome-positive/Ph~+/Ph like T-ALL(Ph~+/Ph like T-ALL)using bioinformatics methods,and analyze the core sub-networks to explore the development process of Ph~+/Ph like T-ALL and find molecular targets that may be used in clinical diagnosis and treatment.Methods:Collecting the WES/RNAseq examination results of Ph~+/Ph-like T-ALL children in our hospital,download the genetic data that meets the requirements from the GEO database,and using the GRO2R online differentially expressed gene screening program to screen for differentially expressed genes,and comparing differentially expressed genes Perform GO function enrichment analysis and KEGG pathway analysis.At the same time,using STRING database and Cytoscape software to build protein interaction network,and screen out hub genes and core sub-networks.Result:Ph+/Ph like T-ALL found a total of 84 differentially expressed genes,Ph+ALL found a total of 249 differentially expressed genes,and T-ALL found a total of 175 differentially expressed genes.Based on the results of GO function enrichment,KEGG pathway enrichment analysis and protein interaction network results,RPA1,POLD1,POLE,and SOCS1are selected as hub genes.DNA damage repair and JAK/STAT signal transduction pathway are the main functional sub-networks selected.Conclusion:There are obvious abnormal DNA damage repair pathways in children with Ph~+/Ph like T-ALL.RPA1,POLD1,POLE may be important relevant biomarkers for the occurrence and development of Ph~+/Ph like T-ALL,which can provide a basis for further research.