| Objective To explore the clinical efficacy of He’s Bushen Houmo Decoction in thin endometrium,and provide objective data for the current thin endometrial treatment field;further study the molecular mechanism of He’s Bushen Houmo Decoction in the treatment of thin endometrium through animal experiments.Methods 1.Thirty cases of thin endometrium patients who met the diagnostic criteria of the subject were selected from the outpatient department of traditional Chinese medicine and gynecology of Hangzhou Traditional Chinese Medicine Hospital.After following the informed consent of the patients,they entered the clinical study.The first day after the menstrual cycle ended,they started taking He’s Bushen Thick Film Recipe,stopped taking it during the menstrual period,and continued to take it for 3 cycles.Follow up and fill in the observation form to count the endometrium before and after treatment.Thickness,endometrial morphology and blood supply,syndrome score,clinical efficacy and other data results.After processing the data,analyze the curative effect of He’s Bushen Thick Film Recipe in clinical application.2.48 female SD rats were selected and randomly divided into 4 groups:Bushen Houmofang group,aspirin group,model group and blank group,with 12 rats in each group.Except for the blank group,the blood supply to both sides of the ovarian arteries was blocked for 20 min.A rat thin endometrial model was established.After one estrous cycle,the model group and the blank group were given normal saline by gavage at a fixed time every day,and the rest were given aspirin and Bushen Houmofang by gavage according to the grouping conditions.The total gavage duration was 15 days.RT-PCR technology was used to detect the expression of PI3K,AKT and internal reference genes.The result Clinical research:1.Before the treatment,the endometrial thickness of the tested patients was 5.30±0.79mm,and after the cycle of He’s Bushen Thick Film Decoction,the endometrial thickness was 7.80±1.63mm,P<0.001.The endometrial thickness after treatment was significantly higher than that before treatment.2.In this experiment,menstrual duration,menstrual volume,and menstrual color were used as TCM syndrome score evaluation indicators.Before the treatment,the TCM syndrome score of the test patients was 10.133±6.41,and after the treatment,the TCM syndrome score was 5.733±5.03,P<0.001,and the TCM syndrome score decreased significantly after the treatment.3.In this experiment,the subendometrial blood supply and endometrial morphology were used as the evaluation indexes of uterine artery blood flow integral.Before treatment,the test index score of patients was 12.200±2.70,and after treatment,the test index score was 9.367±2.88,P<0.001.There was a statistical difference in the test index score before and after treatment.Experimental Study:1.The expression of AKT and PI3K in the endometrial tissue of rats with thin endometrium decreased,and their occurrence and development may be related to the PI3K/AKT signaling pathway.2.Bushen Houmo Recipe and aspirin can improve the expression of AKT and PI3K in the endometrial tissue of thin endometrium rats to a certain extent,P<0.05,the results are statistically different,the effect of Bushen Houmo Recipe group is slightly better than that of aspirin group.Conclusion Compared with the blank group,the expressions of AKT and PI3K in the endometrial tissue of the rats in the model group were significantly decreased,P<0.05;compared with the model group,the expressions of AKT and PI3K in the endometrial tissue of the rats in the Bushen Houmofang group and the aspirin group were significantly increased,P<0.05,and the effect of Bushen Houmofang group was slightly better than that of aspirin group,P<0.05,the difference was statistically significant. |
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