Effect Of Resveratrol On Mitochondrial Energy Metabolism In Exercise-fatigued Rats

Objective:To explore the effect of resveratrol supplementation on mitochondrial energy metabolism in exercise-induced fatigue rats and its molecular biological mechanism,and to provide a theoretical basis for the application of resveratrol in the field of exercise.Method:Forty-eight 6-8 week old SD male rats were randomly divided into blank control group（group C）,resveratrol group（group R）,exercise group（group E）and exercise+resveratrol group（group ER）,12 rats in each group.The ER group performed 6 weeks of weight-bearing 5%swim training at the same time as the E group,60 minutes each time,6 days a week.The ER group was given resveratrol 50 mg/kg by gavage 1 h after exercise,and the R group was given resveratrol 50 mg/kg by gavage.Group C and E were fed normally and the same volume of 2%dimethyl sulfoxide solvent gavage was performed daily.The kit detects plasma BUN content,CK activity,gastrocnemius MD A content,SOD,ATPase,SDH,CS and COX activity.RT-PCR was performed to detect SIRT1,PGC-1α,NRF1 and NRF2 mRNA expression in gastrocnemius muscle tissue.Result:（1）Compared with group C,BUN content and CK activity in plasma of group E were significantly increased（p<0.05,p<0.05）.Compared with the E group,the BUN content and CK activity in the plasma of the ER group were significantly decreased（p<0.01,p<0.05）;Compared with group C,SOD activity in skeletal muscle of group E was significantly decreased（p<0.05）;compared with group E,SOD activity of skeletal muscle in group ER was extremely significantly increased（p<0.01）;Compared with the E group,the MDA content in the skeletal muscle of the ER group was significantly decreased（p<0.05）.（2）Compared with group C,skeletal muscle Ca2+-Mg2+-ATPase,Na+-K+-ATPase,SDH,and CS activity were significantly lower in group E（p<0.01,p<0.05,p<0.05,p<0.05）;compared with group E,skeletal muscle Ca2+-Mg2+-ATPase,Na+-K+-ATPase,SDH,and CS activity were increased in group ER significantly（p<0.01,p<0.05,p<0.05,p<0.05）,and the COX content in the skeletal muscle of rats in group E increased compared with group C（p<0.05）.（3）The mRNA expression of SIRT1 was extremely significantly higher in groups E and R compared to group C（p<0.01,p<0.01）;the mRNA expression of SIRT1 was significantly higher in group ER compared to group E（p<0.15）.The mRNA expression levels of PGC-la were significantly higher in groups E and R compared to group C（p<0.05,p<0.05）;the mRNA expression of PGC-1α was significantly higher in group ER compared to group E（p<0.05）.The mRNA expression levels of NRF1 were significantly higher in groups E and R compared with group C（p<0.05,p<0.05）.The mRNA expression of NRF1 was significantly higher in the ER group compared to the E group（p<0.01）.The mRNA expression of NRF2 was significantly higher in the ER group compared to the E group（p<0.01）.Conclusion:Resveratrol supplementation can promote mitochondrial production by activating the SIRT1-PGC-1α-NRP1 pathway,increase the activity of enzymes related to mitochondrial energy metabolism,improve mitochondrial energy metabolism,improve the body’s antioxidant capacity,and promote the recovery of exercise-induced fatigue.