Comparative Study On Structural Characteristics Of Dietary Nutrients And Intestinal Microflora In Patients With Depression Before And After Treatment

Objective:The 24-hour dietary review survey was carried out for patients with depression before and after treatment to calculate the intake of dietary nutrients and find the association between dietary nutrients and disease.The diversity and structural differences of intestinal microbiota between depressive patients before treatment and healthy people and pre-treatment depressive patients and post-treatment depressive patients were compared,and specific intestinal microbiota related to the disease was searched.It is hoped that this study can find new targets for the treatment of patients with depression in the future.Methods: This study included 44 patients with major depressive disorder in the inpatient Department of Shandong Daizhuang Hospital from January 2021 to October 2021 as the experimental group.The study group was divided into pre-treatment depression group and post-treatment depression group according to 4 weeks of treatment.Forty-one healthy volunteers from surrounding communities served as a control group.General demographic questionnaire,Hamilton Depression Scale,Hamilton Anxiety Scale and other information were collected by questionnaire.A 24-hour dietary review survey was collected from pre-treatment depression group and post-treatment depression group,and dietary nutrients were analyzed by dietary nutrition software.Fecal samples were collected from the control group,pre-treatment depression group and post-treatment depression group,and high-throughput sequencing was performed by 16 S r RNA gene sequencing technology to compare the diversity and structural differences of intestinal flora between the groups.Results:1.The survey results of general demographic characteristics showed that the study group and the control group had no statistical significance in terms of gender,age,height,weight,BMI,years of education,and smoking.2.The rate of fat in total energy was 14.5% in the pre-treatment depression group higher than that of 10.55% in the post-treatment depression group.The rate of carbohydrate in total energy was 66.03% in the pre-treatment depression group lower than that of 72.65% in the post-treatment depression group.Niacin,Vitamin A,vitamin E,magnesium,manganese,zinc and other micronutrients in the pre-treatment and the post-treatment depression group were lower than recommended nutrient intake.The intake of vitamin B1,magnesium,manganese and other micronutrients in the pre-treatment male depression group was higher than that before treatment,but the intake did not reach the recommended intake.The intake of micronutrients such as vitamin B1,vitamin B2,magnesium,zinc and manganese in the pre-treatment female depression group was higher than that before treatment,which did not reach the recommended intake.3.The diversity analysis of intestinal flora between the pre-treatment depression group and the control group showed no significant difference in α-diversity index analysis,while the β-diversity analysis showed a certain degree of difference.There were no significant differences inα-diversity and β-diversity between pre-treatment and post-treatment depression group.4.The relative abundance of Enterobacteriaceae,Actinobacteria increased,while that of Bacteroidetes decreased in pre-treatment depression group.Compared with the pre-treatment depression group,the post-treatment depression group had fewer branches such as Proteobacteria,Enterobacteria,actinobacteria and more branches such as Ruminococcus,Megamonas and Adolescentis.Conclusion:1.Changes in the intake of dietary nutrients in patients with depression may be beneficial to the recovery of the disease.2.Bacteroidetes,proteobacteria and actinomycetes may be the specific flora of depressive patients.3.The relative abundance of certain intestinal flora changes after antidepressant treatment.