Differential Analysis Of Lung Transcriptome In Mice Exposed To Long-term E-cigarettes Or Conventional Cigarettes

Objective:The widespread use of e-cigarettes is a growing public health concern.The aim of this study was to investigate the effects of long-term exposure to electronic cigarettes or conventional cigarettes on the transcriptome of lung tissue in mice.Methods:Thirty C57BL/6 mice were randomly divided into normal control group,traditional cigarette group and electronic cigarette group with ten mice in each group.The traditional cigarette group and electronic cigarette group were given passive smoking for 6 months under the same conditions.Test mice lung function.Collect the lung tissue of mice,part of the lung tissue were used to make the pathological slice for HE dyeing observation of changes in the lung tissue cells in mice;In addition,transcriptome analysis and bioinformatics analysis were performed for lung tissues,and differential genes were screened for GO and KEGG pathway function enrichment analysis.Results:Compared with the normal control group,both the traditional cigarette exposure group and the e-cigarette exposure group showed decreased lung function indexes:FEV0.05(P=0.0068)and FEV0.05/FVC(P=0.0457)in the traditional cigarette exposure group.FEV0.05(P<0.0001),FVC(P=0.0013)and FEV0.05/FVC(P=0.0248)in the e-cigarette exposure group.Pulmonary pathology in mice suggested that the alveolar cavity of both the traditional cigarette exposure group and the e-cigarette exposure group had obvious dilatation and inflammatory cell infiltration.Transcriptomics analysis revealed 1089 differentially expressed genes in the traditional cigarette group and 1044 differentially expressed genes in the e-cigarette group.Differential gene function enrichment analysis showed that in both the traditional cigarette group and the e-cigarette group,the differential genes were mostly distributed in the cytoplasmic organelle structure of cells,Biological processes are mainly clustered in biological regulation,cell transformation,developmental process,metabolic process,multibiological process and stress response.Molecular functionology mainly focuses on the molecular functionology processes of adhesion and catalytic activity,and functions mainly through signaling pathways such as transport and metabolism,signal transduction,translation and immune system.Conclusion:Long-term e-cigarette exposure can lead to the decline of lung function and lung tissue alveolar cavity expansion,inflammatory cell infiltration,resulting in changes in lung structure;Transcriptome analysis could provide a theoretical basis for the potential mechanism of lung function and pathological changes caused by e-cigarettes。...