| Objective: To research the situation of the p53 gene mutation in exon 7,8in crowds at the areas of high incidence of hepatocellular carcinoma in Guangxi;to study the relationship between the mutations of exon 7,8 of p53 gene and familial aggregation of hepatocellular carcinoma in high incidence area of Guangxi;to clarify the role of p53 gene mutation in exon 7,8 in HCC pathogenesis.Methods:From March 2014 to January 2015,over ten areas were selected in high incidence of hepatocellular carcinoma in Guangxi.And 123 cases were respectively selected from families which have had two or more HCC patients,and families which have had only one HCC patients,and families which have no HCC patients so far.All of the families were selected from the high incidence of HCC areas of Guangxi.And 30 cases of HCC patients were collected from high incidence of hepatocellular carcinoma as a control group.HBV infections,HCV infections and environmental factors were controlled as confounding factors.Polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP)was used to screen the mutations of exon 7,8 of p53 gene.Then combined with gene sequencing method to determine the mutation specific circumstances.Results:The HCC patients,group was found three cases of p53 gene mutation in exon 7 and one case of p53 gene mutation in exon 8 by the combination of PCR-SSCP analysis and gene sequencing.The mutation rate of exon 7,8 of p53 gene in the HCC patients,group was 13.3%(4/30).There was no mutations were found in exon 7,8 of p53 gene among all the family subjects.The mutation rate of exon 7,8 of p53 gene in the three family groups was 0%.There is significant difference between the family group and the HCC patients,group(P<0.05).Conclusion:P53 gene mutations in exon 7,8 may not be the genetic susceptibility factors of familial aggregation of hepatocellular carcinoma in high incidence area of guangxi.The mutations of exon 7,8 of p53 gene in high incidence of hepatocellular carcinoma in Guangxi may not be obtained genetic,but acquired under the influence of a variety of factors involved in the occurrence and development of HCC. |
