Correlation Analysis Of MUS81 Gene Polymorphisms And EBV Related Tumors

Epstein-Barr virus(EBV),also known as human herpesvirus 4(HHV-4),is a double stranded DNA virus,which belongs to the lymphocryptovirus genus of the subfamily of gamma type herpesvirus.Studies have confirmed that EBV is the pathogen of infectious mononucleosis(IM).EBV has also been confirmed to be closely related to the occurrence and development of a variety of human malignant tumors,such as Burkitt’s lymphoma,Hodgkin’s lymphoma,NK/T-cell lymphoma,nasopharyngeal carcinoma(NPC),gastric cancer and other epithelial tumors.The carcinogenic effect of EBV is mainly achieved by inducing DNA damage,interfering gene expression and corresponding biological behavior of host cells.MUS81 plays an important regulatory role in the repair of damaged DNA and normal DNA replication,and may participate in the occurrence and development of EBV related tumors.Objective: To investigate the relationship between single nucleotide polymorphisms of MUS81 gene with gastric cancer,NPC,and lymphoma.Materials and methods: The genotypes of rs13817,rs648732 and rs659857 of MUS81 gene in gastric cancer,nasopharyngeal carcinoma,lymphoma tissue and normal control were detected by time of flight mass spectrometry(TOF-MS).Results:(1)The genotype distribution of rs13817 of MUS81 gene in gastric cancer group,EBVa GC group and control group was statistically significant(P < 0.05),but there was no significant difference between EBVn GC group and control group(P > 0.05).In the recessive model of A allele,the gastric cancer group,EBVa GC group and EBVn GC group had statistical significance compared with the control group(P < 0.05),but there was no statistical difference between EBVa GC group and EBVn GC group(P > 0.05).In the dominant model of A allele,there was no significant difference among the groups(P >0.05).There was significant difference in allele frequency between EBVa GC group and control group(P > 0.05).(2)There was no significant difference in rs13817 genotypes of MUS81 gene between NPC group,EBV positive NPC group,EBV negative NPC group and control group,and between EBV positive NPC group and EBV negative NPC group(P > 0.05).In the recessive model of A allele,there was no significant difference between NPC group,EBV positive NPC group and EBV negative NPC group and control group(P >0.05).There was no significant difference in allele frequency between the above groups(P > 0.05).(3)There was no significant difference in rs13817 genotype distribution between EBV positive lymphoma and control group.There was no significant difference in recessive,dominant model and allele frequency of A allele(P > 0.05).(4)There was no significant difference in rs648732 of MUS81 gene among gastric cancer group,ebvagc group,ebvngc group and control group,and between EBVa GC and EBVn GC groups(P > 0.05).In the recessive and dominant models of T allele,there was no significant difference among the above groups(P > 0.05).There was significant difference in allele frequency between ebvagc group and control group,but there was no significant difference in other groups.(5)There was no significant difference in rs648732 genotype distribution among NPC group,EBV positive NPC group,EBV negative NPC group,control group,EBV positive NPC group and EBV negative NPC group(P > 0.05).There was no significant difference in the recessive,dominant model and allele frequency of T allele among the above groups(P > 0.05).(6)There was no significant difference in rs648732 genotype distribution among lymphoma group,EBV positive Lymphoma Group,EBV negative Lymphoma Group and control group,and between EBV positive and EBV negative lymphoma groups(all P >0.05).There was no significant difference in the recessive,dominant model and allele frequency of T allele among the above groups(P > 0.05).(7)The rs659857 locus of MUS81 gene had no significant difference between the gastric cancer group and the control group,but there was significant difference between the EBVa GC group and the control group and the EBVn GC group(P < 0.05),while there was no significant difference between the EBVn GC group and the control group(P >0.05).In the recessive model of T allele,there was no significant difference between the GC group and the control group,but there was significant difference between the EBVa GC group and the control group,and there was no significant difference between the EBVn GC group and the control group(P > 0.05).In the dominant model of T allele,there was significant difference between EBVa GC group and control group(P < 0.05),but there was no significant difference among other groups(P > 0.05).There was significant difference in allele frequency between ebvagc group and EBVn GC group,but there was no significant difference in other groups(P > 0.05).(8)The rs659857 locus of MUS81 gene in NPC and EBV positive NPC groups was significantly different from that in the control group(P < 0.05),but there was no significant difference in other groups(P > 0.05).In the recessive model of T allele,there were significant differences between NPC group and EBV positive NPC group and the control group(P < 0.05),while there were no significant differences among the other groups.In the dominant model of T allele,there were no significant differences among the groups(P > 0.05).Allele frequency analysis showed that there were significant differences between NPC group,EBV positive NPC group and control group,while there were no significant differences among other groups(P > 0.05).(9)There were significant differences in rs659857 of MUS81 gene between lymphoma group,EBV positive and EBV negative lymphoma groups and control group,but there was no significant difference between EBV positive and EBV negative lymphoma groups(P > 0.05).In the recessive model of T allele,there were significant differences between lymphoma group,EBV positive and negative Lymphoma group and control group,but there was no significant difference between EBV positive and negative Lymphoma group(P > 0.05).There was no significant difference among the groups in the dominant model.The allele frequency showed that there were significant differences in lymphoma group,EBV positive lymphoma group and EBV negative lymphoma group compared with the control group(P < 0.05),but there was no significant difference between EBV positive lymphoma group and EBV negative lymphoma group(P > 0.05).Conclusion: the rs13817,rs648732 and rs659857 polymorphisms of MUS81 gene are associated with tumorigenesis and latent infection of EBV in gastric cancer,nasopharyngeal carcinoma and lymphoma.