Detection Of IL-26 In Serum Of Patients With HBV-ACLF And Its Clinical Significance

Objective: To explore the possible role of IL-26 in the pathogenesis of HBV-ACLF patients and to explore its clinical significance,by measuring the level of interleukin-26(IL-26)in the serum of patients with hepatitis B virus-associated acute-on-chronic liver failure(HBV-ACLF).Methods: Thirty patients were collected with HBV-ACLF diagnosed in Linyi People’s Hospital between January 2018 and December 2019 as the experimental group,30 patients with chronic hepatitis B(CHB)as the control group,and 20 healthy subjects as the blank control group.Serum IL-26 levels were detected by enzyme-linked immunosorbent assay(ELISA).Liver function was detected by automatic biochemical analyzer.Prothrombin activity(PTA)was measured by START4 semi-automatic blood coagulation analyzer.Endotoxin was determined by gel-method limulus test.Hypersensitive C-reactive protein(Hscrp)was determined by emulsion agglutination turbidimetric method.Real-time fluorescence quantitative PCR was used to detect HBV-DNA quantification.Hepatocentesis specimens of CHB were collected and stained with HE,histopathological changes were observed under light microscope.Experimental data were analyzed by SPSS21.0 software.Measurement data were expressed as mean ± standard deviation((x±s).The t test is used to test two independent samples.Wilcoxon rank sum test was used to test the non-normal distribution data of the two groups.IL-26 levels were compared with AST,ALT,TBIL,PTA,endotoxin and Hscrp levels by Pearson correlation analysis.Results :1、The level of serum IL-26 in HBV-ACLF group was significantly higher than that in CHB group(t =7.4,p <0.05).In CHB group,the level of serum IL-26 in severe inflammation group was higher than that in mild inflammation group(p <0.05).2、Comparison between subgroups in patients with HBV-ACLF:In patients with HBVACLF,IL-26 level in patients with advanced stage was significantly higher than that in patients with early stage(t =4.3,p <0.05);Secondary bacterial infections interleukins 26 levels were significantly higher than those without having been infected with the bacteria(t =3.5,p <0.05).The level of interleukin 26 was significantly higher in the death group than in the improved group(t=3.2,p <0.05).3 、 Results of Spernman correlation analysis between IL-26 level and biochemical indexes,PTA,Hscrp,and endotoxin in HBV-ACLF patients: there was no significant correlation between the levels of IL-26 and AST,ALT and TBIL(r =-0.201,p >0.05;r =0.278,p >0.05;r =0.101,p >0.05).The level of IL-26 was significantly negatively correlated with PTA(r =-0.668,p < 0.01).The level of IL-26 was significantly positively correlated with Hscrp(r =0.38,p <0.05).The level of IL-26 was significantly positively correlated with endotoxin(r =0.709,p < 0.01).Conclusion :1、The expression level of IL-26 was significantly increased in serum of HBV-ACLF,which was involved in the pathogenesis of HBV-ACLF.2、IL-26 was positively correlated with endotoxin and hypersensitive C-reactive protein,and could be used as a marker of inflammation.