Association CUB And Sushi Multiple Domains(CSMD1)Gene Polymorphisms And Susceptibilities To Idiopathic Parkinson’s Disease In Northern Chinese Han Population

Objective:Previous studies have revealed that the CUB and Sushi Multiple Domains(CSMD1)gene is related to familial Parkinson’s disease.Evidence have shown that the CSMD1 gene is an inhibitor of the complement activation pathway and is also involved in central nervous system inflammation.This study aimed to investigate the relationship of CSMD1 gene single nucleotide polymorphisms(SNPs)with susceptibility to Parkinson’s disease in population of Northern China.Methods:A case-control study was performed with 423 Parkinson’s disease patients and 465 healthy controls matched for age and sex.DNA from enrolled subjects were extracted from the peripheral blood,and SNPs of rs12681349(C>T),rs10503253(C>A)and rs1983474(T>G)within CSMD1 gene were genotyped using polymerase chain reactions-restriction fragment length polymorphism(PCR-RFLP).SPSS21.0 statistical software was used for statistical analysis,and t test of two independent samples was used for measurement data.Counting data were tested by chi-square test.The correlation between genotype and Parkinson’s disease was analyzed by logistic regression.Relative risk is represented by odds ratio(OR)and 95%confidence interval(95%CI).P<0.05 indicated a statistically significant difference.In addition,subgroup analysis was performed based on age of onset and gender.PS Power and Sample Size Software were used to calculate statistical performance.False-Positive Report Probability(FPRP)is used to verify positive results.Results:Genotype frequency of rs10503253(CA vs.CC:OR=1.554,95%CI=1.169-2.066,p=0.002)and rsl983474(GG vs.TT:OR=0.599,95%CI=0.401-0.895,p=0.012)were significantly different between PD cases and controls,but not for rs12681349.Comprehensive and subgroup analysis indicated that rs10503252 showed significant statistical differences in the dominant model(AA+CA vs.CC:OR=0.677,95%CI=0.517-0.886,P=0.004),late-onset cohort(CAvs.CC:OR=1.570,95%CI=1.159-2.126,p=0.004)and the female cohort(CA vs.CC:OR=0.687,95%CI=0.497-0.952,p=0.023),compared with the matched control group.The difference of recessive model of rs1983474(GG vs.TT+TG:OR=1.837,95%CI=1.287-2.620,p=0.001)was significant in Parkinson’s disease.According to the subgroup analysis,results indicated that late-onset cohort(GG vs.TT:OR=0.643,95%CI=0.420-0.985,p=0.042),male cohort(TG vs.TT:OR=2.160,95%CI=1.162-4.016,p=0.015)and female group(GG vs.TT:OR=0.418,95%CI=0.234-0.746,p=0.003)of rs1983474 were significantly associated with Parkinson’s disease susceptibility.Both in genotype and subgroup analysis,we failed to find any relationship between rs12681349 polymorphism and Parkinson’s disease risk.Conclusion:Our results indicate that the rs10503253 and rs1983474 gene polymorphism may be associated with idiopathic Parkinson’s disease susceptibility in Chinese population.Nevertheless,these conclusions need to be further verified by more studies.