Expression Of Thymidine Kinase 1 In Diffuse Large B-cell Lymphoma And Its Clinical Significance

Objective: To analyze the relationship between serum thymidine kinase 1(TK1)levels and clinical features in patients with diffuse large B cell lymphoma(DLBCL)and its effect on efficacy and survival in patients with DLBCL.Research methods: Apply enzyme immune chemical radiation assay to detect the blood serum TK1 level of 32 patients with DLBCL admitted to Department of Hematology,Shengjing Hospital of China Medical University from 2017-1-1 to2020-5-31.Collect complete clinical data and then analyse the relationship between serum TK1 level and clinical features,as well as its effect on efficacy and survival time.Kaplan-Meier method is used for survival analysis,Log-rank test is used for univariate analysis,and Cox proportional hazards regression model is used for multivariate analysis.Results: 1.Univariate logistic regression shows that serum TK1 expression is correlated with clinical stage and lactate dehydrogenase(LDH)level,with statistical significance(P< 0.05).But it is not correlated with bulky disease,B symptom,bone marrow infiltration,serum β2 microglobulin,Myc expression,Bcl2 expression and Ki67level(P > 0.05).Multivariate result shows that TK1 is significantly associated with LDH.2.Fisher’s exact probability method is used to compare the complete response rate and total response rate between TK1 positive and negative groups.The results show that there is no statistical difference in the complete response rate(12.5% vs.25.0%,P=0.646)and the total response rate(62.5% vs.37.5%,P=0.252)between the two groups.3.Univariate analysis results show that TK1 is not related to OS and PFS in DLBCL patients,while BMI,Myc/Bcl2 co-expression,bone marrow infiltration,B symptom,lymphocyte count at diagnosis are correlated with OS.Myc/Bcl2co-expression,B symptom and LDH are correlated with PFS(P<0.05),while other variables have no statistical significance on OS and PFS.4.Multivariate studies show that BMI(HR=0.097,95%CI 0.01-0.94,P=0.044)and B symptom(HR= 3.937,95%CI 1.032-89.537,P=0.045)are independent risk factors for OS in DLBCL patients.Myc/Bcl2 co-expression(HR=6.987,95%CI 1.451-33.65,P=0.015)and B symptom(HR= 4.258,95%CI 1.044-17.371,P=0.043)are independent risk factors for PFS.5.Co-detection of serum TK1 and LDH is risk factor of PFS in patients.Conclusion: There is a significant correlation between serum TK1 level and LDH in DLBCL,but no correlation between TK1 level and efficacy or survival time of DLBCL patients.Co-detection of serum TK1 and LDH is risk factor of PFS.