| Objective:To investigate the effect of Jinguoweigang capsule on regulating the expression E-cadherin through Notch4/Snail pathway in precancerous cancer(PLGC)animal experiments,and further clarify the mechanism of Jinguoweikang capsule in treating PLGC.Methods:In this study,60 rats were randomly divided into model group and blank group.PLGC rat model was established by MNNG synthesis method.After 12 weeks,3 rats were randomly selected from the modeling group and 2 rats were randomly selected from the blank group to test whether the modeling was successful.After successful modeling,the remaining rats in the modeling group were randomly divided into Jinguoweikang group,vidasin control group and modeling group.Drug administration intervention for 8 weeks:Janguoweikang group and vitacase control group were given relevant drugs by gavage,model group was given normal saline by gavage,and blank group rats continued to be fed normally.In the end,all rats were killed,samples were collected and HE staining was performed to check the pathological status of each group.The protein levels and gene expressions of Notch4,Snail and E-cadherin in gastric mucosa were detected by Western blot and q PCR.Results:1.It was shown by HE staining : Gastric tissue structure of blank group was normal without hyperemia,necrosis and inflammatory infiltration.In addition,the gastric glands are complete,clear and closely arranged with clear nucleoli and uniform cytoplasm.In the PLGC model group,the nuclei were hyperchromatic,deformed and morphological change.The number of glands was reduced and the morphology was irregular.Inflammatory cells,goblet cell metaplasia and lymphatic follicular formation were observed.In Jinguoweigang group,PLGC status was improved,mucosa was basically complete,atypical hyperplasia was mild or not obvious and intestinal metaplasia was reduced.In the improvement of PLGC cell morphology,Jinguowekang group had a stronger effect than Vidasein group.2.Western blot results:(1)Compared with the blank group,the expression of Notch4 and Snail in the model group were increased,while the expression of E-cadherin was significantly decreased.(2)Compared with the model group,the values of Notch4 and Snail were down-regulated in the Jinguoweicang group and the vidasin group,while the expression of E-cadherin was more abundant.(3)In terms of Notch4,the Jinguoweikang group was lower than the vitaminase group,indicating that Jinguoweikang capsule could more effectively reduce the expression of Notch4 protein.In terms of Snail expression,the down-regulation effect of Jinguowikang group was stronger than that of the vidasin group,and there was a significant difference.As far as E-cadherin is concerned,the statistical report indicated significant differences between the Jinguoweigang group and the vecasin group.3.qPCR results:(1)Compared with the blank group,Notch4 in the model group was significantly increased;Compared with the model group,Jinguoweikang group was significantly decreased;Compared with Jinguoweigang group,the decrease of Notch4 was weaker in vitacase group.(2)Compared with the blank group,Snail gene expression in the model group was increased with statistical significance.Compared with the model group,there was no difference in Snail expression between the Jinguoweikang group and the vecasin group.However,from the perspective of numerical values,Snail in the Jinguoweikang group and the vecasin group was down-regulated and the effect of Jinguoweikang capsule was more significant than that of the vecasin group.(3)Compared with the blank group,E-cadherin m RNA was significantly decreased in the model group.Compared with the model group,E-cadherin both the Jinguoweikang group and the vidasin group are increased,.Conclusion: Jiguoweigang capsule may reverse PLGC by reducing the expression of Notch4 and inhibiting the expression of downstream target gene Snail,thereby upregulating the expression of E-cadherin and improving the pathological morphology of gastric mucosa in rat PLGC model. |
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