| High-fat diet is closely related to the occurrence of obesity,and can also have a certain impact on other aspects of human body functions,such as changing the structure of intestinal flora and thus having a certain impact on the ecological environment in the intestinal tract.It has been reported that celastrol can play a good role in weight loss by acting on leptin targets,but the hepatorenal toxicity of celastrol and its effect on intestinal flora at the dose of weight loss have not been reported in the literature.This paper mainly studied the effects of celastrol on body weight,blood lipid,liver function,kidney function,intestinal flora and short-chain fatty acids in obese mice induced by high fat diet,and analyzed the effects of changes in intestinal flora diversity and short-chain fatty acid content on body weight,blood lipid and other parameters in mice.Furthermore,the possible mechanism of celastrol on weight loss and lipid reduction in mice fed high fat diet by changing intestinal flora was analyzed.The main research contents of this paper are as follows:1.Analysis of body weight of mice on high fat diet.Through the comparison of high fat diet and normal diet and the influence of celastrol on the body weight of mice on high fat diet was analyzed through the intervention of celastrol.It was found that celastrol could inhibit the weight gain induced by high fat diet in mice with a significant dose effect.According to the analysis of blood lipid indexes in mice,celastrol can significantly improve the blood lipid indexes in mice treated with high fat diet.2.Through the analysis of liver function and kidney function,it was found that the four indexes of alanine aminotransferase(ALT),aspartate aminotransferase(AST),blood urea nitrogen(BUN),creatinine(Cr)had no significant difference compared with the control group.The tissue section analysis of liver and kidney of mice showed that the tissue morphology of liver and kidney was normal,without inflammatory cell infiltration,and there was no significant difference between different treatments,indicating that celastrol did not produce significant toxic effects on liver and kidney of mice under the dose of administration in this experiment.3.Short chain fatty acids in the intestinal tract of mice were analyzed.High fat diet can significantly reduce the contents of short chain fatty acids(acetic acid,propionic acid and butyric acid)in intestinal tract of mice.In the high-fat diet,the contents of short-chain fatty acids in the intestines of mice treated with celastrol were significantly lower than those in the control group,and the reduction of short-chain fatty acids in the intestines of mice was dose-dependent with the administration of celastrol.There was no significant change in the normal diet.4.Genomic DNA of mouse feces was extracted to construct a gene library,and intestinal flora diversity was sequenced and analyzed by α diversity analysis and β diversity analysis.The results showed that celastrol treatment could reduce the richness of Bacteroidaceae and Prevotellaceae in the intestinal tract,and increase the richness of Odoribacter and Ruminiclostridium.In addition,the abundance of Alloprevotella was reduced to a certain extent.The effects of celastrol on intestinal flora were correlated with the changes in the content of short-chain fatty acids.Celastrol inhibited the enrichment of short-chain fatty acid producing bacteria(such as Prevotellaceae)in the intestines,and further affected the production of short-chain fatty acids,resulting in the decrease of shortchain fatty acid content in the intestines of mice.There was no obvious change in the intestinal structure of mice in the analysis of intestinal tissue sections.Therefore,through the analysis of the intestinal flora of mice,it was further clarified that celastrol can affect the physiological and biochemical indexes of mice on high fat diet by regulating the diversity and richness of intestinal flora. |
