The Roles Of HMGB1,TLR4,IL-1β In Epileptogenesis

Objective By detecting the expression of serum HMGB1,TLR4 and IL-1β in patients with epilepsy,to explore the possibility and feasibility of HMGB1,TLR4 and IL-1β as biomarkers for diagnosis,treatment and severity evaluation of epilepsy,so as to improve the understanding of them and provide basis for clinical diagnosis and prevention in the future.Methods 1.The peripheral blood of 31 patients with epilepsy and 30 healthy volunteers were collected,and the data of age,sex,disease status,course of disease,seizure frequency and average seizure time were collected at the same time.2.The levels of serum HMGB1,TLR4 and IL-1β in 31 patients with epilepsy and 30 healthy volunteers were measured by enzyme-linked immunosorbent assay((ELISA)).3.The experimental group was divided into different subgroups according to the course,frequency and average duration of seizures.4.The levels of serum HMGB1,TLR4 and IL-1 β in different subgroups were compared.Results Our results showed that HMGB1,TLR4,IL-1β expression levels were significantly higher in epilepsy patients than that in control subjects(2.59 ± 0.49 ng/ml vs.1.75 ± 0.35 ng/ml,P < 0.0001;2.10 ± 0.30 ng/ml vs.1.11 ± 0.29 ng/ml,P < 0.0001;18.80 ±5.83 pg/ml vs.11.21 ± 3.16 pg/ml,P < 0.0001;respectively).The results also illustrated that expression levels of serum HMGB1 and TLR4 in patients with epilepsy were positively correlated(r=0.6838,P<0.001).The results from receiver operating characteristic curve(ROC)analysis indicated that the expression levels of serum HMGB1,TLR4 and IL-1β in patients with epilepsy could be a specific and sensitive potential diagnostic biomarker.Conclusion Our results suggested that the serum HMGB1,TLR4 and IL-1β could be a specific and sensitive potential diagnostic biomarker for epilepsy as the expression levels of serum HMGB1,TLR4 and IL-1β in patients with epilepsy may be related to the severity of epilepsy.