| Objective: To explore the role of specific nuclear matrix binding domain binding protein 1(SATB1)and micro RNA(micro RNA,mi R)-495-3P in the invasion and metastasis of papillary thyroid carcinoma(PTC).Methods: All fresh specimens were collected from the Thyroid and Breast Surgery Department of the Second Affiliated Hospital of Fujian Medical University from September 2019 to April 2020.Among them,40 cases of papillary thyroid carcinoma and 40 cases of normal paracancerous thyroid tissue were corresponding.Real-time quantitative reverse transcription polymerase chain reaction(RT-q PCR)was used to detect the expression levels of SATB1 and mi R-495-3p in 40 pairs of papillary thyroid carcinoma tissues and normal thyroid tissues adjacent to the cancer.Western blot was used to detect 16 pairs of thyroid papillary carcinoma tissues and normal thyroid tissue adjacent to the SATB1 protein expression levels,and analyzed the relationship between the expression of SATB1 and mi R-495-3p and the clinicopathological characteristics of papillary thyroid carcinoma.After transfecting human papillary thyroid carcinoma cells(TPC)-1 with si-SATB1,RT-q PCR and Western blot were used to detect the expression levels of SATB1 and mi R-495-3p in TPC-1,and Pearson The analysis method was used for correlation analysis,in which TPC-1 transfected with si-NC was the negative control group,and TPC-1 transfected with si-SATB1 was the experimental group.After transfection,the cell counting kit(CCK-8)method,flow cytometry method,and Transwell method were used to detect the effect of knockdown SATB1 expression on the proliferation,apoptosis,cycle,invasion and migration of TPC-1.The t test was used to compare the two samples.Results: Compared with normal thyroid tissue adjacent to cancer,SATB1 m RNA and SATB1 protein were highly expressed in papillary thyroid carcinoma tissues(1.27±0.14 vs.0.86±0.23,t=8.484,p<0.01 and 0.94±0.10 vs.0.37±0.15,respectively,T=11.890,p<0.01),the difference was statistically significant.The expression of mi R-495-3p was low(0.78±0.11 vs.1.37±0.64,t=5.741,p<0.01),and the difference was statistically significant.The abnormal expressions of SATB1 and mi R-495-3p are significantly correlated with lymph node metastasis of papillary thyroid carcinoma(1.34±0.14,t=2.576,p<0.05 and 0.74±0.07,t=2.187,p<0.05),and age,Gender,tumor size,number of lesions,and extraglandular invasion were not significantly related.The expression levels of SATB1 and mi R-495-3p in papillary thyroid carcinoma were negatively correlated(r=-0.497,p<0.01).Interfering with the expression of SATB1 in TPC-1 will cause the expression level of mi R-495-3p to be higher than that of the negative control group(8.59±0.16 vs.1.01±0.02,t=81.420,p<0.01),and inhibit cell proliferation(0.39± 0.01,0.52±0.01,0.58±0.03 than0.43±0.01,0.60±0.01,0.72±0.01,t=4.899,9.789,7.668,p<0.01),promote cell apoptosis [(42.8±2.1)% ratio(7.6± 0.7)%,t=27.540,p<0.01],reduced cell invasion(75.33±3.51 vs.206.33±5.51,t=34.730,p<0.01),migration(202.00±7.81 vs.528.33±5.03,t=60.840,p< 0.01)Ability and occurrence of G2/M cycle block[(36.7±1.2)% to(15.6±0.9)%,t=24.360,p<0.01),the difference was statistically significant.Conclusion:1)SATB1 is down-regulated in PTC,mi R-495-3p is up-regulated in PTC,and the abnormal expression of SATB1 and mi R-495-3p is significantly related to PTC lymph node metastasis.2)The expression levels of SATB1 and mi R-495-3p in PTC are negatively correlated(r=-0.497,p<0.01).Interference with the expression of SATB1 in TPC-1 will increase the expression level of mi R-495-3p.3)Interfering with the expression of SATB1 in TPC-1 can inhibit cell proliferation,promote cell apoptosis,weaken cell invasion and migration,and cause G2/M cycle block. |
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