| Background:Esophageal carcinoma is a malignant tumor of the digestive tract that originates from the epithelial cells of the esophagus.It is one of the cancers that threaten life and health globally.Esophageal cancer is divided into two categories: esophageal squamous cell carcinoma(ESCC)and esophageal adenocarcinoma(EAC),of which esophageal squamous cell carcinoma is the main histological subtype.circular RNAs(circ RNAs)are a special type of RNA.In most cases,reverse splicing forms a continuous loop with covalent closure from the 5’-to the 3’-end.Researchers have been discovered clinically significant circ RNA and reverse RNA that are related to linear non-coding RNA(nc RNA),indicating that these circ RNAs may be related to diseases.Recent studies have shown that some particular circ RNAs can act as post transcriptional regulators by competing with m RNAs for binding to a large number of micro RNAs(mi RNAs)and / or RNA binding proteins(RBPs).In our previous study,37 ESCC and adjacent tissues were collected,and circ＿0006168 were detected in ESCC and normal tissues specimens by quantitative real-time PCR(q RT-PCR).It was shown the relative expression of circ＿0006168 in ESCC tissue samples was significantly higher than that in normal tissues;the expression of micro RNA(mi R)-384 in ESCC tissue specimens was relatively decreased;and the expression of retinoblastoma binding protein 7(rbbp7)was relatively increased.Objective:In this study esophageal cancer cells ECA-109,KYSE-510 and human normal esophageal epithelial cells HET-1A were used as models,to preliminarily study the corresponding regulatory relationship between circ＿0006168 、 mi R-384 and RBBP7,and their roles in proliferation of esophageal cancer cells.Methods:In this study,q RT-PCR was used to measure circ＿0006168,mi R-384 and RBBP7 m RNA expression in cancer and normal cells;protein expression of RBBP7 was analyzed by Western blot;after interfering or enhancing the expression of circ＿0006168,mi R-384 and RBBP7,q RT-PCR was performed to detect the changes of the three,and the regulatory relationship of the three was analyzed;the MTT method was used to detect the proliferation level of cancer cells;and the lactic acid detection kit was used to detect lactic acid expression of cancer cells after transfecting circ＿0006168、mi R-384 and their inhibitors.Results:The data showed that the overexpression of circ＿0006168 improved the proliferation rate of esophageal cancer cells,and the proliferation rate of esophageal cancer cells decreased after interfering with mi R-384.Si RNA interference assay found that low expression of circ＿0006168could lead to lower lactate content in ESCC cells,and interference with circ＿0006168expression might negatively regulate glycolysis and exert inhibitory effects on the proliferation process of ESCC cell S.Essentially,the results of the present study suggested that circ＿0006168was a sponge of mi R-384,and RBBP7 was a target of mi R-384.circ＿0006168 promoted RBBP7 m RNA and protein expression by inhibiting mi R-384 in esophageal cancer cells.In addition,inhibition of mi R-384 expression decreased circ＿0006168 silencing mediated inhibition of cell proliferation.Overexpression of RBBP7 relieved the inhibitory effect of mi R-384 on the proliferative capacity of esophageal cancer cells Conclusion:In conclusion,this study shows that circ＿0006168 enhanced the glycolytic pathway of esophageal squamous cell carcinoma cells to obtain energy by regulating mi R-384/RBBP7,thereby promoting the proliferation of esophageal squamous cell carcinoma cells.Our findings preliminarily clarified the mechanism of circ＿0006168 in the proliferation of esophageal squamous cell carcinoma.Moreover,this study also provided an alternative candidate for the treatment of esophageal squamous cell carcinoma,and enriched more experimental clues for further research on the mechanism of esophageal carcinoma and screening of early diagnostic markers. |
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