The Study On The Expression Of Ribosomal Protein In Septic Myocardial Injury

Sepsis is a systemic multi-organ tissue inflammatory response syndrome caused by inflammation and infection,and it is a common clinical emergency and severe disease.Sepsis often cause various organ damages,such as cardiac insufficiency,pulmonary edema,acute respiratory distress syndrome,gastrointestinal bleeding,liver damage,and acute renal failure.Patients may have abnormal myocardial function in the early stage of sepsis,including a variety of cardiovascular dysfunction and cardiac insufficiency,and their internal mechanisms are very complex and affect each other.Ribosomal protein(RP)is an important part of the ribosome.In recent years,many studies have shown that in addition to its role in protein production,RP also plays a role in the occurrence and development of diseases.However,it plays a role in sepsis,and the role in this is still unclear.Mitochondrial damage and abnormal energy metabolism are important pathways leading to myocardial damage in sepsis.Mitochondrial ribosomes(MRP)play an important role in maintaining the normal function of mitochondria.Therefore,we speculate that MRP is related to the occurrence of myocardial damage in sepsis Has a certain role in development.In our study of the expression of two ribosomal proteins in septic myocardial injury,we provide a reference for clinical septic myocardial injury.Methods: In this experiment,60 clean-grade C57/BL adult mice,weighing 26～30g,were randomly divided into normal group(Control group),intraperitoneal injection of endotoxin group(LPS group),and intraperitoneal injection of cecal contents solution group(CS group).In the early stage of the experiment,all mice were under gas anesthesia for echocardiographic observation to ensure that the heart function of each mouse was normal.After the model was constructed,when the mice showed obvious septic shock,the echocardiographic observations were performed to screen out the mice with obvious myocardial dysfunction,their heart tissues were taken out,transcriptome sequencing was performed,and the blood was taken from the chest cavity while the tissues were taken.Separate mouse plasma for detection of myocardial injury markers.By analyzing the results of transcriptome sequencing,we obtained the gene expression of septic myocardial injury mice.We screened out ribosomal genes with abnormal expression in the LPS and CS experimental groups,designed primers,performed Q-PCR verification,used WB to verify the expression of individual ribosomal proteins,and then used software for data analysis.Results: 1.After septic shock in mice,mice with cardiomyopathy will obviously shorten the length of LVESd and LVEDd,that is,the occurrence of myocardial dysfunction,and the left ventricular ejection fraction will be reduced by nearly 20%.2.The gene function enrichment analysis results of the transcriptome show that genes such as ribosomes and riboprotein complex biogenesis,r RNA processing,ribosomal biogenesis,r RNA metabolism,ribosomal subunits and other genes have little damage in septic myocardium.There is a significant difference in expression in mice.3.The Q-PCR results showed that in the septic myocardial injury mice established by two different methods,the expression of RP increased and the expression of MRP decreased,and the changes were consistent with the sequencing results.4.WB results showed that the expression of mitochondrial ribosomes was reduced,which was consistent with the results of transcriptome sequencing and Q-PCR.Conclusion: After septic shock in mice with septic myocardial injury,the left ventricular ejection fraction decreases.The serum markers BNP and c Tn I are significantly different between the model group and the control group,which can be used as the specificity of myocardial injury in mice.The expression of cytoplasmic RP in septic myocardial injury mice increased,and the expression of MRP decreased,and it began to change in the early stage of septic positive myocardial injury.The abnormalities of the two RPs and transcription and translation effects The abnormality of RP is related,and the regulation of ribosome biogenesis may be enhanced after a series of abnormal RP expression.