The Influence Of Mesoporous Bioactive Glass Composite Scaffolds Adsorbed With Fusion Protein On Osteoblast

Bone defect makes bone the second largest transplanted tissue in the world,so hoping to find an alternative to traditional bone transplantation through bone tissue engineering.Constructing cell scaffolds with excellent biocompatibility,degradability,and mechanical strength remains a challenge in bone tissue-engineering.By virtue of heterologous expression systems,a fusion protein based on Osteocalcin(OCN),osteopontin(OPN)and Biglycan(BGN)was obtained;through sol-gel process and templated self-assembly,a magnesium-doped mesoporous bioactive glass was prepared,which is able to slow-release the aforementioned fusion protein.These two parts,together with polycaprolactone,were electrospun to construct the targeted composite scaffold.In this dissertation,a composite scaffold composed of slow-released fusion protein,mesoporous bioactive glass,and polycaprolactone was prepared to have good biocompatibility and osteogenic activity.In vitro study shows that the proliferation,differentiation and mineralization of mouse osteoblast MC3T3-E1 can be significantly promoted by this scaffold,especially when a 3:1 Ca/Mg ratio of mesoporous bioactive glass adsorbed with fusion protein was applied.This result suggests a decent osteogenic effect of this material,providing preliminary fundamentals for further clinical application in bone-defect repair.