Study On The Protective Mechanism Of Remote Ischemic Preconditioning Combined With Rosuvastatin On Hepatic Warm Ischemia-reperfusion Injury In Rats

Objective: Through the rat liver warm ischemia-reperfusion injury model,study the protective effect of remote ischemic preconditioning and rosuvastatin on hepatic warm ischemia-reperfusion injury.Observe whether the effect is better after the combined application of the two.Provide methods and theoretical basis for clinical prevention and treatment of hepatic warm ischemia-reperfusion injury.Method: Forty healthy SD male rats,6-8 weeks old,were randomly divided into sham operation group(SO),ischemia reperfusion group(IR),remote ischemic preconditioning group(RIPC),rosuvastatin pretreatment group(RS)and RIPC+RS group.Modeling method: Used 70% liver warm ischemia-reperfusion injury model.The hepatic artery and portal vein of the left and middle lobe of the liver were clamped with microvascular clamps,blocked for 1 hour,and reperfused for 2hours.After operation,inferior vena cava blood and liver specimens were taken,serum ALT,AST,TNF-α and IL-1β were detected,MDA and SOD were detected in liver specimens,pathological sections of liver specimens were made and the pathological changes of liver tissues were observed.SPSS22.0 statistical software was used to analyze and process the research data statistically.The data was expressed as mean±standard deviation.The analysis of differences between multiple groups of measurement data used analysis of variance,and the pairwise comparison within the group used LSD test.The defined test level was 0.05,and the difference was statistically significant at P<0.05.Results:(1)Compared with the SO group,the levels of ALT,AST,TNF-α,IL-1β,and MDA in the IR group,RIPC group,RS group and RIPC+RS group were significantly increased,and the SOD level was significantly reduced.In the SO group,the morphology of liver cells was complete,and the degree of liver tissue in the IR group,RIPC group,RS group and RIPC+RS group was obvious.(2)Compared with the IR group,the ALT,AST,TNF-α,IL-1β and MDA levels of the RIPC group and the RS group were significantly reduced,the SOD level was increased,and the liver tissue damage in the group was mild.(3)Compared with the RIPC group and the RS group,the ALT,AST,TNF-α,IL-1β and MDA levels in the RIPC+RS group were significantly reduced,the SOD level was increased,and the liver tissue damage was mild.(4)Pathological results: SO group: Rat liver cells were normal,with clear morphology and structure,and hepatic cords were neatly arranged.IR group: The liver cells of rats were severely damaged,accompanied by cell edema,necrosis,hepatic cord rupture,hepatic sinusoid dilatation and hyperemia in the portal area,accompanied by inflammatory cell infiltration.The rats in the RIPC group and RS group had milder liver cell damage,mild cell edema,disordered hepatic cord arrangement,mild hepatic sinusoid dilation and congestion,and no obvious inflammatory cell infiltration.In the RIPC+RS group,the liver cells were the least damaged,the cells were slightly edema,the hepatic cords were neatly arranged,and there was no obvious inflammatory cell infiltration.Conclusions: Both RIPC and RS had protective effects on liver warm ischemia-reperfusion injury,and the combination of the two significantly enhanced the protective effect on liver warm ischemia-reperfusion injury.The protective mechanism may activate the endogenous protective mechanism and related pathways,thereby reducing ALT,AST,TNF-α,IL-1β,MDA and increasing SOD,reducing inflammation and oxidative stress.