| Background and ObjectiveBrain glioma abbreviation is brain glioma,it is the commonest tumor type in central nervous system,brain glioma is in numerous type the incidence of disease in brain tumor is highest,occupy about 70% of the tumor inside skull above.The median survival of patients with glioblastoma from the discovery of lesions to death is only 8-12 months,despite the treatment of surgery,radiotherapy,chemotherapy and other methods.The effect of the current treatment methods for patients with glioma is relatively limited,and it can even be said that it has reached a bottleneck state.The overall survival time of patients is short,the tumor recurs quickly,and the side effects of radiotherapy and chemotherapy are very prominent.Moreover,patients often appear after surgical treatment of neurological problems,to the patients and their families caused great inconvenience to life.Therefore,there is a special need to find new treatment methods and techniques."Magnetic field therapy for tumor",a new non-invasive treatment method,is emerging with the continuous progress of medical technology,which provides a new idea for the clinical treatment of glioma.Methods1.Use CCK-8 experiment to observe the growth curve of U87 cells treated with50 Hz,20m T LF-MF(Low-Frequency Magnetic Fields,low frequency magnetic field),and analyze the effect of low frequency magnetic field on the proliferation of glioma U87 cells;2.Use Transwell experiment to observe the migration and invasion ability of glioma U87 cells treated with 50 Hz,20m T LF-MF;3.Use flow cytometry technology to examine the apoptosis of glioma U87 cells treated with 50 Hz,20m T LF-MF,and analyze the effect of low-frequency magnetic field on the apoptosis of glioma cells;4.Using Real-time PCR technology to test the expression of various factors that promote and inhibit tumor growth in glioma U87 cells treated with 50 Hz,20m T LF-MF;5.Apply animal experiments to verify the effect of 50 Hz,20m T LF-MF on tumor growth in tumor-bearing nude mice.Results1.Compared with the control group,the proliferation ability of glioma U87 cells after LF-MF treatment at 50 Hz and 20 m T was decreased,with statistical significance(P< 0.05).2.Compared with the control group,glioma U87 cells treated with LF-MF at50 Hz and 20 m T showed decreased migration ability(P<0.001)and decreased invasion ability(P<0.01),which was statistically significant.3.The apoptosis rate of glioma U87 cells treated with LF-MF at 50 Hz and20m T showed no significant difference compared with the control group,which showed no statistical significance.4.Compared with the control group,the expression levels of VEGF,MMP-9,HDAC1,ITGA5 and TRIM44 in glioma U87 cells treated with 50 Hz and 20 m T LF-MF were significantly decreased(P<0.01),while the expression levels of YWHAG and MX2 were significantly increased(P<0.01).5.Glioma U87 cells were implanted subcutaneous into nude mice,and the nude mice were divided into two groups: one group was irradiated by LF-MF at 50 Hz and20m T,and the other group was not irradiated by LF-MF.The results showed that the tumor volume of tumor-bearing nude mice in the irradiated group by LF-MF was significantly reduced compared with the control group.ConclusionThrough the experimental detection and analysis of U87 cells treated with low frequency magnetic field at 50 Hz and 20 m T,it was found that the proliferation ability of glioma cells irradiated by low frequency magnetic field was lower than that of the control group,and the invasion and migration ability was also weaker.In addition,in the subsequent verification of animal experiments,it was found that the tumor growth of the tumor-bearing nude mice treated with low-frequency magnetic field was slower than that of the control group,and the final tumor size was also smaller than that of the control group.The results suggested that the low frequency magnetic field of 50 Hz and 20 m T had inhibitory effect on glioma U87 cells. |
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