| Articular cartilage is an important tissue that supports,cushions and lubricates the joints of the human body.Its basic structure is composed of the surface hyaline cartilage layer,the middle and deep calcified cartilage layer and the subchondral bone layer.Relevant data show that articular cartilage damage has an extremely high proportion of joint diseases.However,natural cartilage is difficult to supply internal nutrients and cartilage cells are weak in proliferation,which makes it difficult to repair and grow after injury.At present,the main methods to deal with cartilage damage are still drug treatment and clinical treatment.The damaged part is difficult to regenerate and repair,the treatment effect is poor,the recovery period is long,and the operation pain is greater.Therefore,artificial research on artificial cartilage has become a hot spot in current research.Currently,artificial cartilage substitute materials that have been put into clinical use are mostly made of plastic,metal or ceramic,and it is difficult to produce a good combination with the bone or cartilage interface after being implanted in the body.At the same time,its corrosion resistance and other issues still need to be resolved.The above-mentioned problems of conventional cartilage replacement materials have made the research on joint osteochondral damage repair through tissue engineering gradually become the main direction of current research.In response to the above problems,this paper designed and prepared an integrated bionic hydrogel cartilage repair material to solve the appeal problem,which includes two functional hydrogel layers,which are used for the cartilage layer and the subchondral bone layer.The main research results are as follows:(1)The PVA/PAA high-strength hydrogel material is prepared by penetrating PAA,which can better meet the repair needs of the cartilage layer.Among them,the tensile strength and tensile elastic modulus of 12% PVA/PAA Cold-Drawn hydrogel reach to 14.54 ± 0.89 MPa and2.67 ± 0.47 MPa,and the compressive strength and compressive elastic modulus at 80% strain reach to 22.04 ± 0.67 MPa and 2.12 ± 0.11 MPa.It also has good cell compatibility,which can promote cell growth and adhesion on the surface.(2)PAM cross-linked network hydrogel was prepared by using PAM and MBA as raw materials.Based on it,PAM-Mineralized hydrogel was further prepared,and the surface of the hydrogel has a stable mineralized structure.Analysis of the mineralization structure shows that the main component of the mineralization structure is hydroxyapatite.The mechanical properties test show that the mineralization process does not significantly affect the mechanical properties of PAM-Mineralized hydrogels,and its mechanical properties are basically the same as those of PAM hydrogels.At the same time,the presence of the mineralized structure effectively improves the cells of PAM-Mineralized hydrogels.Compatibility allows MC3T3-E1 cells to adhere and grow well on the surface,which is conducive to their combination with subchondral bone.ALP test results show that PAM-Mineralized hydrogel has good osteogenic differentiation ability.(3)Combining the characteristics of PVA/PAA Cold-Drawn hydrogel and PAM hydrogel,PVA/PAA-PAM integrated hydrogel is prepared through integrated molding.The hydrogel has good integrity and effectively solves the difficulty of single-component hydrogel to meet cartilage repair needs.Through the integrated molding method,the interfacial layer of the usual multi-level hydrogel structure does not exist in the hydrogel,which effectively solves the shortcoming of easy displacement between the multi-level hydrogel layers. |
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