Study On The Chemical Constituents Of Fuzi And Aconitum Stapfianum

Natural products(NPs)remain an important source of drug research,and it is of great significance to study their chemical constituents.As one of the important Chinese medicinal materials in Aconitum,the lateral root of Aconitum carmichaelii(Fuzi)is widely used in a variety of diseases,but improper use can lead to serious poisoning,which is mainly manifested in cardiotoxicity,neurotoxicity,embryonic toxicity and renal toxicity.However,Aconitum stapfianum as the same genus of Fuzi,has been used for a long time to detoxify Fuzi.Therefore,the purpose of this project is to study the chemical constituents of Fuzi and Aconitum stapfianum,establish research strategies for the bioactive alkaloids in Aconitum stapfianum for the poisoning of Fuzi and explore the material basis of its detoxification.In this study,two new alkaloids and eighteen known alkaloids were isolated and purified from Fuzi by column chromatography and liquid chromatography.Then,the content determination method of alkaloids was established.The linear relationship and the stability were satisfactory.By comparing the contents of 17 alkaloids in Fuzi,the main root of Aconitum carmichaelii(Wutou),Baifu tablet and Heishun tablet,the distribution of toxic components and pharmacodynamic components in raw materials and processed products was obtained,and the content of toxic components was significantly reduced after processing.In the research process of Aconitum stapfianum,a strategy was developed by intensely understanding the fragment pathway of authentic standards.A series of rules were summarized,such as charge site effects,hydrogen bonding effects,competitive channels between charge-remote reaction and charge migration reaction,and fragment pattern of “diamond shape”.Accordingly,a total of 124 alkaloids were tentatively characterized in A.stapfianum,including 85 potential new compounds and 24 sets isomers.On another hand,to primarily explore the material foundation of detoxification,chemical constituent comparison was made between A.stapfianum and Fuzi,and principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were performed for identifying the markers.Moreover,25 characteristic markers were identified to discriminate these two herbal medicines,of which 14 compounds were specific for A.stapfianum and most of them were characteristic for substituted benzoic acid ester at C-14.Compared with antiarrhythmic drugs,it is preliminarily believed that the structure of benzene ring replaced by oxygen-containing groups may have the effect of antiarrhythmic aconite poisoning.