Evaluation Indexes Of Immune Function In Patients With Colorectal Cancer Based On NK Cell Protein Profile

Objective:To investigate the characteristics of different receptor expression profiles in patients with colorectal cancer and the differences between them and healthy controls.Methods:The peripheral blood samples were collected from 144 patients with colorectal cancer and 87 healthy individuals,and the expression of both peripheral blood receptors on T lymphocytes,natural killer（NK）cells and NKT cells on colorectal cancer were analyzed by flow cytometry.The killing function of NK cells in patients with colorectal cancer was investigated by cell culture,peripheral blood mononuclear cell isolation and CD107a expression level.Results:Compared with healthy controls,the proportion of CD16^brightCD56^dimNKcell surface activating receptor NKG2D in colorectal cancer patients has an upward trend;the proportions of T cells,CD16^brightCD56^dimNK cells,and NKT surface inhibitory receptor TIGIT are all higher than those in healthy controls,and the expression of TIGIT Increased with the increase of pathological stage;CD16^brightCD56^dimNK cells,NKT cell surface chemokine receptor CCR7 ratio has a downward trend compared with healthy controls,and the expression of CCR7 decreased with the increase of pathological stage;NK cell CD107a and healthy Compared with the control,there is no significant difference.Conclusion:This study reveals that the surface inhibitory receptor TIGIT and the chemokine receptor CCR7 in colorectal cancer patients may be related to the occurrence,development and even prognosis of colorectal cancer,providing potential targets for immunotherapy of colorectal cancer patients.Meanwhile,the activating receptor NKG2D on the surface of CD16^brightCD56^dimNK cells varies with the pathological stage,which will become the basis for different pathological stages of colorectal cancer in the future and the use of different immunotherapy methods.