Analysis Of GEO Database To Explore The Difference Between Primary Central Nervous System Lymphoma And Diffuse Large B Cell Lymphoma

Objective:The pathogenesis of primary central nervous system lymphoma(PCNSL)is still unclear.Its clinical manifestations are specific to the central nervous system.There is no significant difference between PCNSL and n DLBCL in histology.Based on the microarray analysis of the whole gene expression profiles of PCNSL and n DLBCL,we explored the differences between them from the perspective of genetics,and further studied the molecular mechanism of the disease,looking for potential diagnostic and therapeutic targets.Methods:In the Gene Expression Omnibus(GEO)database,“primary central nervous system lymphoma”was used as the key word to search and download the data set GSE11392.There were 43 GSM,including 13 PCNSL,11 n DLBCL and 19 extranodal DLBCL from other tissues,13 PCNSL and 11 nodal DLBCL samples were analyzed by“limma R”package.For the up-regulated or down regulated genes,GO function analysis,KEGG signaling pathway analysis and protein interaction network analysis were performed using“cluster Profiler R”package.The differentially expressed genes were analyzed in combination with the results of ct DNA detection in peripheral blood of patients with PCNSL.Results:After data analysis,we found 240 differentially expressed genes(DEGs)between PCNSL and n DLBCL,including 139 up-regulated genes and 101 down regulated genes.The differentially expressed genes involved 349 GO entries and 12 KEGG pathways,especially PI3K-Akt signaling pathway.Interaction network analysis combined with signal pathway analysis showed that CCR5,TLR2,C5AR1 and GNB3 played a key role.ct DNA analysis showed that ITPKB was detected in peripheral blood of patients with PCNSL.Conclusion:The pathogenesis of PCNSL involves different genes and pathways.By analyzing the gene expression profiles of PCNSL and n DLBCL,we found that the pathogenesis of PCNSL may be caused by the interaction between tumor cells and glial cells,neurons and the microenvironment of central nervous system.SPP1,TCL1 A,CHI3L1,CCR5,TLR2,C5AR1,GNB3 can be used as potential diagnostic markers.PI3K-Akt signaling pathway plays an important role in the occurrence of PCNSL.PI3 K inhibitors can be used to treat PCNSL.The correlation between ITPKB gene and PCNSL remains to be verified.