Construction Of Poly (ionic Liquids) Hydrogel With Rapid Antibacterial Function And Its Application In Wound Healing

As the area of human activities continues to expand,various diseases caused by bacterial infections are also increasing,which threat people’s health and life seriously.Therefore,it is necessary to develop antibacterial agent for meeting people’s requirements.Since the invention of antibiotics in the last century,scientists have successively developed different types of antimicrobial agents,and then antimicrobial materials developed rapidly and applied widely.However,the development of treatment of wound healing cannot meet clinical requirements owing to the complex bacteria-infection and inflammatory reaction.If the skin is damaged due to an accident,the resulting wound infection may seriously threaten the life and health of the injured.In the process of skin wound healing and reorganization,biomedical dressings can temporarily function as part of the skin barrier,and provide a favorable microenvironment for skin wound healing and increase the speed of wound healing by releasing drugs.It is worth noting that bacterial infection of the wound can cause severe inflammation.A large amount of exudate at the wound can decompose extracellular matrix proteins and a variety of growth factors,which will lead to prolonged wound healing time and increase the difficulty of treatment.Therefore,the development and application of wound antibacterial dressings are of great significance to the treatment of wound healing.So,it is necessary to develop wound dressings that are fast antibacterial property,anti-inflammation and promoting cell migration and proliferation for shortening wound healing period.In this work,we first synthesized two poly（ionic liquids）（PILs）:poly（1-ethyl-3-vinylimidazolium furan-2-carboxylate）（PEIF）and poly（1-butyl-3-vinylimidazolium furan-2-carboxylate）（PBIF）.The PILs were then mixed with5 wt%montmorillonite（MMT）clay and 2-furfurylamine-modified hyaluronic acid（HAF）,and chemically crosslinked with four-arm maleimide-polyethylene glycol（Mal₄PEG）to form degradable semi-inter penetrate network（semi-IPN）hydrogels.In vitro assays demonstrated that the synthesized semi-IPN hydrogels not only exhibited high antibacterial activities against Escherichia coli and Staphylococcus aureus due to the high antibacterial activity of PILs,but also had low cytotoxicity.The semi-IPN hydrogel could be quickly degraded,and the PILs exhibited high anti-inflammatory activities when they were released from degraded hydrogels.Furthermore,the degradation solutions of hydrogels contained glycosaminoglycan,which was beneficial to cell proliferation and migration.The in vivo anti-infection results further demonstrated that the semi-IPN hydrogels could kill S.aureus effectively and on the 14th day,the wound healing ratio treated with PHC-BIF hydrogel could reach 80%.