Relationship Between Plasma 8-iso-PGF2α Level And Coronary Artery Lesions With Kawasaki Disease

Objective: To investigate the situation of oxidative stress in children with Kawasaki disease(KD)by measuring plasma 8-iso-prostaglandin F2α(8-iso-PGF2α),and to explore the correlation and predictive value of plasma 8-iso-PGF2α and Coronary artery injury(CAL)in children with KD.Methods: A total of 102 children(CAL n=53,NCAL n=49)in acute stage of KD who were admitted to the Department of Pediatric Cardiology,Lanzhou University Second Hospital from December 2018 to December 2019 were selected as subjects(KD group).A total of 103 children with no discomfort or infection were selected as the healthy control group(HC group).A total of 108 children with respiratory tract infection(n=74 bronchopneumonia,n=34 acute upper respiratory tract infection)admitted to the Second Hospital of Lanzhou University during the same period were selected as the fever control group(FC group).Fastening venous blood was collected in the morning for detection of plasma 8-iso-PGF2α in each group,and clinical data,interleukin-6(IL-6),C-reactive protein(CRP),neutrophil to lymphocyte ratio(NLR)and heart color Doppler ultrasonography were collected in each group.SPSS25.0software was used for data collection and statistical analysis.Results: 1.The plasma levels of 8-iso-PGF2α,IL-6,CRP and NLR in KD group were significantly higher than those in FC group and HC group(P < 0.05),and the plasma levels of 8-iso-PGF2α,IL-6,CRP and NLR in FC group were significantly higher than those in HC group(P < 0.05).2.The plasma 8-iso-PGF2α level,IL-6,CRP and NLR in the CAL group were higher than those in the NCAL group,and the plasma 8-iso-PGF2α level was positively correlated with IL-6,CRP and NLR(correlation coefficients were 0.921,0.686,0.791,P < 0.05).3.Plasma 8-ISO-PGF2αlevel was an independent risk factor for coronary artery injury in children with KD.The best predictive level was 960.985pg/ml,sensitivity and specificity were 69.8%and 95.9%,respectively,and AUC was 0.824(95% confidence interval: 0.734-0.915,P < 0.05).4.Plasma 8-ISO-PGF2α combined with IL-6 was the best predictor of KD combined with CAL,with a predictive cut-off value of 0.5518,sensitivity of 76.9%,specificity of 98%,and AUC of 0.842(95% confidence interval: 0.589-0.793,P <0.05).5.After IVIG treatment,plasma 8-ISO-PGF2α,IL-6,CRP and NLR were significantly decreased in KD group(P < 0.05).Conclusion: 1.The oxidative stress molecule 8-ISO-PGF2α is involved in the occurrence of Kawasaki disease vasculitis.2.IVIG can significantly reduce the level of oxidative stress in children with KD.3.Plasma 8-iso-PGF2α level may be an independent risk factor for coronary artery injury in children with KD,and plasma8-iso-PGF2α combined with IL-6 is one of the best indicators for predicting coronary artery injury in Kawasaki disease.