NLRC5 Promotes Cell Proliferation,migration And Invasion By Activating The PI3K/AKT Signaling Pathway In Ovarian Cancer Cells

Ovarian cancer is a common malignant tumor in gynecological tumors worldwide,with high incidence and high mortality.As a silent female killer,the 5-year survival rate of ovarian cancer patients has basically remained unchanged in the past 20 years.Low level of 35-46%.Although there have been great advances in the exploration of the mechanism of ovarian cancer,the pathogenesis of a large part of its occurrence is still unknown.Therefore,an in-depth understanding of the pathogenesis of ovarian cancer is very important.This in-depth understanding is likely to provide new targets for the treatment of ovarian cancer.According to some current studies,we can know that NOD-like receptor family caspase recruitment domain family-containing 5(NLRC5)plays a vital role in a variety of human malignancies.The purpose of this study is to explore the role of NLRC5 in ovarian cancer cells.Method(1)Collect ovarian cancer tissues and normal ovarian tissues excised by surgical patients in the Department of Obstetrics and Gynecology,Second Affiliated Hospital of Anhui Medical University,and use Western bloting,RT-q PCR and immunohistochemical methods to detect NLRC5 in the corresponding tissues The expression situation.(2)The ovarian cancer cells SKOV3 and HO-8910 were transfected with an overexpressing NLRC5 plasmid,and the expression of NLRC5 in the cells was detected by Western bloting and RT-q PCR.The proliferation ability of SKOV3 and HO-8910 cells was detected by CCK-8 method.The migration and invasion ability of SKOV3 and HO-8910 cells was detected by Transwell method.(3)Using NLRC5-siRNA to knock down the expression of NLRC5 in ovarian cancer cells SKOV3 and HO-8910,and use Western bloting and RT-q PCR to detect the expression of NLRC5 in cells.The proliferation ability of SKOV3 and HO-8910 cells was detected by CCK-8 method.The migration and invasion ability of SKOV3 and HO-8910 cells was detected by Transwell method.(4)LY294002 was used to treat ovarian cancer cells SKOV3 and HO-8910 transfected with NLRC5 plasmid,and Western blotting was used to detect PI3 K,Akt,P-PI3 K,and P-Akt.The proliferation ability of SKOV3 and HO-8910 cells was detected by CCK-8method.The migration and invasion ability of SKOV3 and HO-8910 cells was detected by Transwell method.Result(1)Western bloting、RT-qPCR and immunohistochemistry were used to detect the expression of NLRC5 in ovarian cancer tissues and normal ovarian tissues.The results showed that compared with normal ovarian tissues,NLRC5 expression was lower in ovarian cancer tissues(P <0.05).(2)After ovarian cancer cells SKOV3 and HO-8910 were transfected with NLRC5 plasmid,the results of Western bloting and RT-q PCR showed that the expression of NLRC5 in the overexpression plasmid group was higher than that in the empty plasmid group(P<0.05).CCK-8 experiment results showed that SKOV3 and HO-8910 cell proliferation ability increased after transfection of overexpression plasmid(P<0.05).The results of Transwell experiments showed that the migration and invasion ability of SKOV3 and HO-8910 cells increased after transfection of the overexpression plasmid(P<0.05).(3)After ovarian cancer cells SKOV3 and HO-8910 were transfected with NLRC5-siRNA,the results of Western bloting and RT-q PCR showed that the expression of NLRC5 in the NLRC5-siRNA group was lower than that in the control group(P<0.05).CCK-8 experiment results showed that after transfection of NLRC5-siRNA,the proliferation ability of SKOV3 and HO-8910 cells decreased(P<0.05).Transwell experiment results showed that after transfection with NLRC5-siRNA,the migration and invasion ability of SKOV3 and HO-8910 cells decreased(P<0.05).(4)Western bloting results showed that overexpressed NLRC5 can promote the expression of P-PI3 K and P-Akt in SKOV3 and HO-8910 cells.After LY294002 treated ovarian cancer cells SKOV3 and HO-8910 transfected with NLRC5 plasmid,Western blotting results showed that the expression of P-PI3 K and P-Akt in the LY294002+NLRC5 plasmid group was lower than that in the simple plasmid group,and the CCK-8 experiment results showed overexpression NLRC5 can promote the proliferation of SKOV3 and HO-8910 cells.The proliferation of ovarian cancer cells SKOV3 and HO-8910 in the LY294002+NLRC5 plasmid group is lower than that of the simple plasmid group.Transwell experiment results showed that overexpressed NLRC5 can promote the migration and invasion ability of SKOV3 and HO-8910 cells.The migration and invasion ability of ovarian cancer cells SKOV3 and HO-8910 in the LY294002+NLRC5 plasmid group is lower than that of the simple plasmid group.Conclusion(1)Compared with normal ovarian tissues,NLRC5 m RNA and protein are lower in ovarian cancer tissues.(2)Overexpressed NLRC5 can promote the proliferation,migration and invasion of ovarian cells SKOV3 and HO-8910.(3)The low expression of NLRC5 can inhibit the proliferation,migration and invasion of ovarian cells SKOV3 and HO-8910.(4)NLRC5 can promote the proliferation,migration and invasion of ovarian cells SKOV3 and HO-8910 through the PI3K/Akt signaling pathway.These research results provide a possibility for follow-up research and targeted therapy of ovarian cancer.