Long Non-coding RNA TPTEP1 Modulates GADD45α By Targeting MiR-4295 In AML

ObjectiveThe purpose of this study was to to investigate the long non-coding RNA TPTEP1 regulate the expression of GADD45α in AML by targeting microRNA-4295(miR-4295),thus affecting cell proliferation and apoptosis.MethodsThe quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect TPTEP1 expression levels in AML cells and normal control CD34+.The expression of TPTEP1、miR-4295 and GADD45α in AML cells were changed by transfection plasmid.Cell counting kit-8(CCK-8)and flow cytometry analysis were carried out to detect the effects of TPTEP1 and miRNA-4295 on cell proliferation and apoptosis.The correlation between miR-4295 and TPTEP1 or GADD45α was detected by double luciferase reporter gene assay.The expression of GADD45α was detected by western blotting experiment.All data were statistically analyzed using SPSS 20.0 software.ResultsTPTEP1 was down-regulated in AML samples as well as in AML cell lines.Over-expression of TPTEP1 inhibited AML cells proliferation and promoted cell apoptosis.And we found that silencing miR-4295 produced the similar effect of TPTEP1 over-expression.TPTEP1 regulated the malignant behavior of cells by binding to miR-4295.In addition,over-expression of TPTEP up-regulated the growth arrest and DNA damage inducible alpha(GADD45α),a direct target of miR-4295 which play a suppressive role in AML cells.ConclusionThis work reveals the regulatory mechanism of the lncRNA TPTEP1/miR-4295/GADD45α axis in leukemia cells,which may be one of the keys to exploring effective diagnostic biomarkers and therapeutic strategies.