Study On The Method And Application Of Transforming Hydroxyapatite From Ink Fish Bone

The main inorganic component of human and vertebrate bones is hydroxyapatite.Hydroxyapatite accounts for about 60%of human bone and 97%of tooth bone.Hydroxyapatite has excellent biocompatibility,bioactivity and chemical stability in human pH environment,and is widely used as a repair material for human bone defects.Therefore,it is of great significance to study the preparation process and technology of hydroxyapatite.Cuttlefish bone is a natural biomaterial.The main components of laminar matrix and dorsal shield are aragonite calcium carbonate.Hydroxyapatite was prepared by hydrothermal method at 120℃,150℃and 180℃for 8h/24h.The samples were characterized by XRD,SEM,FT-IR and XPS.The transformation of hydroxyapatite from cuttlefish bone laminar matrix and dorsal shield powder under different experimental conditions was studied.The results showed that under the condition of hydrothermal reaction at 120℃for 8 h,the calcium carbonate in cuttlefish lamellar matrix was almost completely transformed into hydroxyapatite.The aragonite phase calcium carbonate in cuttlefish bone dorsal shield powder was almost completely transformed into hydroxyapatite under the condition of hydrothermal reaction at 180℃for 8 h.Calcite phase calcium carbonate was found in laminar matrix and dorsal shield of cuttlefish,but the content was different.The content of calcite phase calcium carbonate was higher in dorsal shield of cuttlefish.The biocompatibility of hydroxyapatite in simulated body fluid was studied.The results show that the pH value of simulated body fluid will affect the type of products in the deposition process.When the pH value changes from acidic to alkaline,with the increase of the pH value of simulated body fluid,the weight gain ratio of hydroxyapatite prepared by cuttlefish bone laminar matrix is 0.67%,0.88%and 1.03%respectively after soaking in simulated body fluid with pH value of 6.0,7.4 and 8.0 for 7 days;The hydroxyapatite prepared from cuttlefish bone shield was soaked in simulated body fluid with pH value of 6.0,7.4 and 8.0 for 7 days,and the weight gain ratios were 0.58%,0.79%and 0.93%,respectively.The results show that the alkaline condition is more conducive to the deposition of hydroxyapatite.After immersion in simulated body fluid,the weight gain ratio of hydroxyapatite prepared by cuttlefish bone laminar matrix was higher than that prepared by cuttlefish bone dorsal shield,and the pH value of simulated body fluid changed more obviously.The reason was that the pores of hydroxyapatite transformed by cuttlefish bone laminar matrix were more developed and the specific surface area was higher,It is more conducive to increase the contact area with simulated body fluid and the attachment and precipitation of hydroxyapatite.