| Objective:The rat model of high pressure injection injury was established.By detecting the serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),pathological manifestations of liver tissue,tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6),the liver injury and its characteristics after high pressure injection injury were explored to provide theoretical basis for clinical diagnosis and treatment.Methods:Sixty-four healthy adult male SD rats were randomly divided into control group and experimental group.The control group was injected with 20 ml normal saline in a midpoint syringe at the back of the thigh about 3cm above the right knee joint,while the experimental group was injected with the same amount of normal saline with a high pressure spray gun at the same position.Serum levels of ALT,AST,TNF-αand IL-6 were measured at 4 h,6 h,18 h and 24 h.Liver histomathological changes were observed under light microscope and electron microscope.Paired sample t test was performed for comparison between groups,and one-way analysis of variance was performed for comparison at different time points within groups.Results:1.Liver function test results:(1)Serum ALT: the serum ALT of the experimental group was higher than the control group in all periods,and the difference between the two groups at 4hours was not statistically significant(P>0.05).The difference between the two groups at 6 hours was statistically significant(P<0.05).The difference between the two groups at 18 hours was statistically significant(P<0.01).The difference between the two groups at 24 hours was statistically significant(P<0.01).Within the experimental group,the difference between 4 hours and 6 hours was statistically significant(P<0.01).The difference between 4 hours and 18 hours was statistically significant(P<0.01).There was no significant difference between 4 hours and 24 hours(P>0.05).There was no significant difference between 6 hours and 18 hours(P>0.05).The difference between 6 hours and 24 hours was statistically significant(P<0.01).The difference between 18 hours and 24 hours was statistically significant(P<0.01).(2)Serum AST:AST of the experimental group was higher than the control group at each time period,and the difference between the two groups at 4 hours was not statistically significant(P>0.05).The difference between the two groups at 6 hours was statistically significant(P<0.01).The difference between the two groups at 18 hours was statistically significant(P<0.01).The difference between the two groups at 24 hours was statistically significant(P<0.01).Within the experimental group,the difference between 4 hours and 6 hours was statistically significant(P <0.01);The difference between 4 hours and 18 hours was statistically significant(P <0.01).There was no significant difference between 4 hours and 24 hours(P>0.05).There was no significant difference between 6 hours and 18 hours(P>0.05).The difference between 6 hours and 24 hours was statistically significant(P<0.01).The difference between 18 hours and 24 hours was statistically significant(P<0.01).2.The histopathological changes of liver:(1)HE staining of liver: the edema of liver cells was not obvious in the experimental group and the control group at 4 hours;The edema of liver cells in the control group was not obvious at 6 hours.At 6 hours,compared with the control group,the experimental group showed swelling of liver cells,edema of liver cells around the central lobule vein,and infiltration of a few lymphocytes in portal area.In the control group,the liver cells showed flaked edema,some hepatic cords were disordered,and a few lymphocytes were infiltrated in portal area.At 18 hours,the hydrologic degeneration of liver cells in the experimental group was more serious than that in the control group.The cytoplasm was loose and lightly stained,presenting diffuse hydrologic degeneration.The Disse space of a large number of liver cells was expanded,the hepatic cord was disorganized,the structure was unclear,lymphocyte infiltration was increased,and there were sporadic necrosis and apoptotic cells.In the control group,the watery degeneration of hepatocytes was reduced and the structure of hepatic cord was restored at24 hours.At 24 hours,the degree of edema in the experimental group was worse than that in the control group.Some hepatic cord structures recovered,the number of infiltrating lymphocytes decreased,and the number of necrotic and apoptotic cells increased.(2)Hepatic transmission electron microscopy: there was no significant change in liver cells in the control group at 4 hours,while chromatin was pyknosis with a few lipid droplets and lysosomes in the experimental group.At 6 hours,there were a few lipid droplets in the liver cells of the control group,while in the experimental group,mitochondrial edema,ridge rupture and endoplasmic reticulum dilation were observed.In the control group,mitochondria were edema with a few lipid droplets at 18 hours,while in the experimental group,nuclear chromatin was further pyknosis,endoplasmic reticulum was further expanded,cell membrane was ruptured,and organelles entered the peri-sinusoid space.In the control group,mitochondria dilated and lysosomal phagocytosis damaged mitochondria at 24 hours,while in the experimental group,cytoplasm of liver cells was reduced,a large number of organelles remained in the periosinusional space,nuclear pyknosis,and more late apoptotic cells were seen.3.Detection results of inflammatory indexes:(1)Serum TNF-α : The serum TNF-α in the experimental group was higher than that in the control group at all time periods,and the difference between the two groups at 4 hours was statistically significant(P<0.01).The difference between the two groups at 6 hours was statistically significant(P <0.01).The difference between the two groups at 18 hours was statistically significant(P<0.01).The difference between the two groups at 24 hours was statistically significant(P<0.01).In the experimental group,the difference between 4 hours and 6 hours was not statistically significant(P>0.05).The difference between 4 hours and 18 hours was statistically significant(P<0.05).The difference between 4 hours and 24 hours was statistically significant(P<0.01).The difference between 6 hours and 18 hours was statistically significant(P<0.01).The difference between 6 hours and 24 hours was statistically significant(P<0.01).The difference between 18 hours and 24 hours was statistically significant(P<0.01).(2)Serum IL-6:The serum IL-6 in the experimental group was higher than that in the control group at all time periods,and the difference between the two groups at 4 hours was statistically significant(P<0.01).The difference between the two groups at 6 hours was statistically significant(P<0.01).The difference between the two groups at 18 hours was statistically significant(P<0.05).There was no significant difference between the two groups at 24 hours(P>0.05).Within the experimental group,the difference between 4 hours and 6 hours was statistically significant(P<0.01);The difference between 4 hours and 18 hours was statistically significant(P<0.01).The difference between 4 hours and 24 hours was statistically significant(P<0.01).There was no significant difference between 6 hours and 18 hours(P>0.05).The difference between 6hours and 24 hours was statistically significant(P<0.01).The difference between 18 hours and 24 hours was statistically significant(P<0.01).Conclusion:1.High pressure injection injury can cause liver injury in rats.2.Serum TNF-α and IL-6 were involved in liver injury after high pressure injection injury in rats.3.The liver injury of rats was obvious from 6 hours to 18 hours after high pressure injection injury. |
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