Degradation Behavior And Biocompatibility Of Mg-Zn-Y-Nd Alloy Gallbladder In Rabbits

BackgroundClamping cystic duct and cystic artery with ligation clamp is a commonly used method in laparoscopic cholecystectomy.However,if the ligation clip remains in the body for a long time,it will have corresponding effects on the body and may produce artifacts on imaging examination.Therefore,we should continue to search for an ideal bioabsorbable material to make ligation clips in clinical application.ObjectiveThe purpose of this study was to investigate the degradation behavior and biocompatibility of different types of Mg-Zn-Y-Nd alloy materials as gallbladder clamps in rabbits,to explore the feasibility and safety of their ligation clamps,and to evaluate their application value.MethodsThirty-six rabbits were randomly divided into bare metal alloy clamp group(group A),phytic acid coated alloy clip group(group B),microarc oxidation(MAO)/ phytic acid coated alloy clip group(group C)and blank control group(group D),with 9 rabbits in each group.Rabbits in group D were not given operation;the rabbits in the other groups were given open surgery and the gallbladder wall tissues were clamped with sterilized Mg-Zn-YNd alloy clamp with different coating.The general situation of rabbits in the operation group was observed after operation.The serum levels of magnesium ion,calcium ion and indexes of liver function,kidney function were measured.The degradation of alloy clamp in each operation group was observed by X-ray diffraction at 7,14,21 days after operation;the rabbits in each operation group were sacrificed and the alloy clamp was removed at 7,14,21 days after operation to observe the degradation of the alloy clamp by scanning electron microscope.ResultsThe vital signs of rabbits in each group were stable and no complications occurred after operation,The results of X-ray diffraction and scanning electron microscope showed that with the prolongation of postoperative time,the structural defects on the surface of gold clip in group A was the most obvious;the crack of gold clip in group B deepened;only slight cracks could be seen on the surface of gold clip and the structure of gold clip was complete in group C.There was no significant difference in serum levels of magnesium ion,calcium ion and the indexes of liver function,renal function among the four groups at different time points before and after operation(P> 0.05).Conclusions1.Mg-Zn-Y-Nd alloy clips have good biocompatibility,low biological toxicity and high safety in rabbits.2.The degradation rate of MAO/ phytic acid coated alloy in rabbits is lower than that of bare metal alloy clamps,and its biocompatibility is better.